analysis of penicillin-binding protein measurement techniques（分析penicillin-binding蛋白质测量技术）.docVIP

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analysis of penicillin-binding protein measurement techniques（分析penicillin-binding蛋白质测量技术）.doc

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analysis of penicillin-binding protein measurement techniques（分析penicillin-binding蛋白质测量技术）

Analysis of penicillin-binding protein measurement techniques [Paper Keywords] deuterium labeled penicillin-binding protein fluorescent polyacrylamide gel electrophoresis autoradiography [Abstract] At present, the growing problem of bacterial resistance to the clinical treatment of difficult, the β-lactam antibiotics, certain strains of penicillin-binding proteins (PBPs) resulting in resistance to change is the β-lactam characteristics of antibiotics. general bacterial PBPs Currently, the growing problem of bacterial resistance to the clinical treatment of difficult, the ICI phthalocyanine amine antibiotics, certain strains of penicillin-binding protein (PBps) changes caused by drug resistance, the ICI phthalocyanine amine the characteristics of antibiotics Generally bacterial PBPs are cytoplasmic membrane proteins, bacterial lethal target. It’s the number, location, caused by changes in affinity with ICI phthalocyanine amine combination antibiotic resistance is not easy. People often using SDS polyacrylamide gel electrophoresis and autoradiography method PBPs map in order to understand and susceptible strains resistant difference, so, PBPs pressure measurement techniques is to study the acid-amine in an important means of resistance [l].’s penetrating weak, with the general autoradiography PBPs map can not be required to increase the use of fluorescence blinking agent efficient ‘fluorescent autoradiography.’ China reported very little about PBps measurement techniques to 3H less marked correspondent, this article describes the technology. 1 Materials and methods 1.1 Materials 1.1.1 Strains of Streptococcus pneumoniae 31003,30301 (Beijing Pharmaceutical and Biological Products; Pen08.R6 our laboratory strains. 1.1.2 medium C + Y medium, see reference (2). 1.1.3 only devices and drugs Vertical slab gel electrophoresis tank (Wuxian Research Instrument Factory); NG-l vacuum gel dryer (Taicang television factory); TGLL a type 1a desktop high-