凯氏定氮法测定饲料粗蛋白的注意细节（Attention to detail of determination of crude protein in feeds by Kjeldahl method）.docVIP

凯氏定氮法测定饲料粗蛋白的注意细节（Attention to detail of determination of crude protein in feeds by Kjeldahl method） 106570611583505400 attention to detail of determination of crude protein in feeds by Kjeldahl determination methodThe Kjeldahl method of nitrogen determination of protein nitrogen conversion method, standard method, the operation is simple, the determination results of repeatability and reproducibility are good, widely used for determination of protein content in food, grain and feed samples. In order to make the measurement result have better accuracy, accuracy and accuracy, it is very important to have a careful and detailed grasp of each step, details and corresponding precautions. First, the sample grinding The determination requires that the sample be crushed and all over 40 meshes are screened for the purpose of making it homogeneous, easy to dissolve, and easier to digest. But the following four points should be mastered: (1) because the sample is easy to produce automatic grading during crushing or sieving, such as corn, the hard parts are often gathered on it, and the soft parts are gathered below. So the sieved to re mix, in order to reduce the measurement error; (2) after crushing, crushing in always left a small part, this part can not be easily discarded, otherwise it will affect the sample heterogeneity; (3) the grinding process to be fast, to avoid the composition change of samples; (4) for the full price of feed (even the full price of feed particles) and concentrated feed, do not need to be crushed, of course this is based on good mixing uniformity of the machine; if it will be crushed, lost part of powder material, and crushed after grading, but reduces the sample heterogeneity. Two, the amount of sample The amount of sample according to the circumstances, the standard provisions of the law is 0.5 ~ 1g, the amount of the sample can be concentrated feed, fish meal and soybean meal with high protein, such as rice, corn and other low prote