accumulating microglia phagocytose injured neurons in hippocampal slice cultures involvement of p38 map kinase积累小胶质细胞吞噬受伤神经元在海马切片文化的参与p38激酶地图.pdfVIP

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accumulating microglia phagocytose injured neurons in hippocampal slice cultures involvement of p38 map kinase积累小胶质细胞吞噬受伤神经元在海马切片文化的参与p38激酶地图.pdf

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accumulating microglia phagocytose injured neurons in hippocampal slice cultures involvement of p38 map kinase积累小胶质细胞吞噬受伤神经元在海马切片文化的参与p38激酶地图

Accumulating Microglia Phagocytose Injured Neurons in Hippocampal Slice Cultures: Involvement of p38 MAP Kinase 1. 1. 1,2. 2 Takahiro Katayama , Hayato Kobayashi , Toshiyuki Okamura , Yuko Yamasaki-Katayama , 1 3 4 4 1 Tatsuya Kibayashi , Hiroshi Kimura , Keiko Ohsawa , Shinichi Kohsaka , Masabumi Minami * 1 Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan, 2 Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan, 3 Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan, 4 Department of Neurochemistry, National Institute of Neuroscience, Tokyo, Japan Abstract In this study, microglial migration and phagocytosis were examined in mouse organotypic hippocampal slice cultures, which were treated with N-methyl-D-aspartate (NMDA) to selectively injure neuronal cells. Microglial cells were visualized by the expression of enhanced green fluorescent protein. Daily observation revealed microglial accumulation in the pyramidal cell layer, which peaked 5 to 6 days after NMDA treatment. Time-lapse imaging showed that microglia migrated to the pyramidal cell layer from adjacent and/or remote areas. There was no difference in the number of proliferating microglia between control and NMDA-treated slices in both the pyramidal cell layer and stratum radiatum, suggesting that microglial accumulation in the injured areas is mainly due to microglial migration, not to proliferation. Time-lapse imaging also showed that the