桑色素对小鼠T淋巴细胞体外活化、 增殖及细胞周期影响.docVIP

桑色素对小鼠T淋巴细胞体外活化、 增殖及细胞周期影响 　 作者：臧宁, 曾耀英, 黄秀艳, 王通, 叶雪仪, 周建国, 王会营 毕业论文 【关键词】; 桑色素;,T细胞活化;,增殖;,细胞周期 毕业论文 　　The effect of morin on activation, proliferation and cellcycle of murine T lymphocytes in vitro 毕业论文 　　[Abstract]; AIM: To discover the effects of morin on the activation, proliferation and cellcycle of murine T lymphocytes in vitro. METHODS: Murine lymph nodederived T lymphocytes were separated and stimulated with concanavalin A (ConA) and different experimental groups were set by cocultured with morin of different final concentration. Flow cytometry (FCM) was used to detect the activation, proliferation [carboxylfluorescein diacetate, succinimide ester (CFDASE) staining] and cellcycle [propidium iodide(PI) staining] of T cells. RESULTS: After 6 h time of culture in vitro, the; rate of CD69+ T cells in control group was (2.97±0.12)%, while it was significant higher in ConA group[(72.52±0.66)% (Plt;0.01)]. Morin could downregulate this rate at final concentration being 25, 50 and 100 μmol/L, with a peak at 100 μmol/L morin[(48.95±0.81)% (Plt;0.01)]. CFDASE staining showed that at 48 h and 72 h, the proliferation indexes (PI) of T cells in ConA group were (1.58±0.04) and (1.95±0.02), respectively. Morin could significantly decrease the PI value at all experimental concentration, with the peak effect at 100 μmol/L morin, which the PI for 48 h was (1.02±0.02) and (1.03±0.01) for 72 h (Plt;0.01). FCM analysis of PI staining implied that the percentage of S phase cells in ConA group was (27.05±0.39)%, significantly higher than that in control group (5.10±0.07)%; and the 25 and 50 μmol/L morin groups showed higher S phase cell rates. CONCLUSION: Morin can significantly inhibit ConA stimulated activation and proliferation of murine T lymphocytes, in which the S phase lagging may serve as one of the major mechanisms. 毕业论文 　　[Keywords]morin; T cells activation; proliferation; cell cycle 毕业论文 　　[摘 要]; 目的: 研究桑色素(morin)对小鼠T淋巴细胞活化、 增殖和细胞周期的影响。方法: 以刀豆蛋白A(ConA)