氧化型低密度脂蛋白对骨髓内皮前体细胞增殖、凋亡及NO分泌功能.docVIP

氧化型低密度脂蛋白对骨髓内皮前体细胞增殖、凋亡及NO分泌功能 　作者：宗小娟，王海昌，周祥，程康 【关键词】 内皮祖细胞 Effect of oxidized lowdensity lipoprotein on proliferation, apoptosis, and NO secreting function of endothelial progenitor cells from bone marrow 　　【Abstract】 AIM: To study the effect of oxidized lowdensity lipoprotein(oxLDL) on the proliferation, apoptosis, and NO secreting function of endothelial progenitor cells(EPCs). METHODS: Bone marrow derived EPCs were isolated from bone marrow blood of swine by density gradient centrifugation, then oxLDL of different concentrations (group B, 5 mg/L; group C, 10 mg/L; group D, 20 mg/L) were added into culture medium to detect the effect of oxLDL on EPCs. MTT assay was used to detect the effect of oxLDL on the multiplication ability of EPCs. Flow cytometry was used to detect the apoptosis caused by oxLDL. Then NO content was measured in the cell culture medium by nitrate reductase method to find the injury of oxLDL on cells and cell functions. RESULTS: Compared with that in the control group, oxLDL inhibited the proliferation of EPCs （group B, 0.189±0.007; group C, 0.112±0.008; group D, 0.070±0.008） （n=8）, promoted the apoptosis of EPCs （group B, 18.35±0.08; group C, 24.22±0.07; group D, 40.37±0.13） （n=6）, and damaged the NO secreting function of EPCs. These effects were intensified with the increasing concentration of oxLDL (Plt;0.001). CONCLUSION: OxLDL can inhibit the proliferation of EPCs, damage the NO secreting function and promote the apoptosis of the cells. It had been seen that EPCs was scant in the blood of coronary artery disease patients. There may be some relationship between oxLDL and the deficiency of EPCs. 　　【Keywords】 endothelial progenitor cells; lipoproteins, LDL; nitric oxide 　　【摘要】 目的： 探讨氧化低密度脂蛋白（oxLDL）对骨髓内皮前体细胞（EPCs）增殖、凋亡及一氧化氮(NO)分泌功能的影响. 方法： 密度梯度离心法获得猪的骨髓EPCs，不同浓度oxLDL(B组5 mg/L，C组10 mg/L，D组20 mg/L）作用于EPCs，MTT法检测oxLDL对EPCs增殖能力的影响，流式细胞仪检测oxLDL对细胞的凋亡率的影响，硝酸还原酶法检测细胞培养液中NO含量的变化，观察oxLDL对细胞及其功能的影响. 结果： 与对照组相比，oxLDL抑制EPCs增殖（B组0.189±0.007，C组0.112±0.00