脓毒症大鼠有核细胞PPARγ活性变化对TNF-α及IL-4影响.docVIP

脓毒症大鼠有核细胞PPARγ活性变化对TNF-α及IL-4影响 　 作者：黄晓梅， 曾勉， 卢桂芳， 关开泮， 黄书然 【摘要】 【目的】 观察大鼠脓毒血症时有核细胞过氧化物酶体增殖物激活受体γ (PPARγ)活性、血浆促炎介质TNF-α及抗炎介质IL-4的变化，探讨其关系。 【方法】 雄性SPF级SD大鼠90只，体质量280 ～ 300 g，随机分为正常对照组、假手术组、脓毒血症组，每组再分12 h、24 h、48 h三亚组，每组10只。采用盲肠结扎穿孔(CLP)制作大鼠脓毒血症模型，ELASA方法测定有核细胞的PPARγ活性及血浆中IL-4的水平变化趋势。【结果】 脓毒血症组大鼠血浆促炎介质TNF-α与正常对照组和假手术组相比升高明显，分别为(162 ± 32 vs 31 ± 7， 32 ± 8) pg/mL，随时间延长有上升趋势，在术后24 h达高峰，后逐渐下降，但仍然处于较高水平状态。抗炎介质IL-4水平也明显较正常对照组和假手术组升高(分别为89 ± 25 vs 41 ± 7， 41 ± 7) pg/mL，并呈持续性升高(70 ± 5， 75 ± 8， 122 ± 11) pg/mL，存在促炎和抗炎平衡失调。脓毒血症组大鼠有核细胞PPARγ活性与正常对照组和假手术组相比明显降低，分别为(0.263 ± 0.017， vs 0.292 ± 0.005， 0.294 ± 0.007)并随病情加重而逐渐下降。脓毒血症组大鼠有核细胞PPARγ活性与血浆促炎介质TNF-α水平呈负相关， r = -0.655，P = 0.040。 【结论】 脓毒血症大鼠存在炎症-抗炎系统失衡，有核细胞PPARγ活性下降，且与血浆促炎介质TNF-α呈负相关。 【关键词】 过氧化物酶体增殖物激活受体; 炎症介质; 脓毒血症 Abstract： 【Objective】 To observe the relationship between the peroxisome proliferator activated receptor γ (PPARγ) activity in nucleated cells and pro-inflammatory mediator tumor necrosis factor α (TNF-α)， anti-inflammatory mediator IL-4 in plasma of sepsis rats. 【Methods】 Ninety male SD rats weighing 280-300 g at specific pathogen free (SPF) level were randomly divided into three groups， which were control group， sham operation group， and sepsis group. And every group was further divided into 12 h， 24 h， and 48 h subgroups， every subgroup containing 10 rats. Sepsis model of rats were established by the operation of caecal ligation and puncture. The PPARγ activity in nucleated cells and pro-inflammatory mediator TNF-α， anti-inflammatory mediator IL-4 in plasma were detected by ELASA. 【Results】 Compared with control group and sham operation group， the TNF-α level was increased in sepsis group (162 ± 32 pg/mL vs 31 ± 7 pg/mL and 32 ± 8 pg/mL， respectively)， and the peak level appeared in 24 h. Compared with control group and sham operation group， the IL-4 level was increased in sepsis group (89 ± 25 pg/mL vs 41 ± 7 pg/mL and 41 ± 7 pg/mL， respectively) and the increment was maintained during the experiment (