重组人睫状神经因子（rhCNTF）短期应用对谷氨酸钠肥胖大鼠血脂.docVIP

重组人睫状神经因子（rhCNTF）短期应用对谷氨酸钠肥胖大鼠血脂 　 作者：郭淑芳，韩国柱，李卫平，周勤 【摘要】 目的 研究短期应用rhCNTF对谷氨酸钠肥胖大鼠血脂的影响。方法 建立MSG大鼠模型：出生d1的SD大鼠，雌雄兼用，皮下注射谷氨酸钠（MSG）3mg/（g·d），连续5天，正常对照组皮下注射等容积生理盐水。3月龄时将肥胖动物随机分5组：分别皮下注射rhCNTF300μg/（kg·d）（高剂量组）、100μg/（kg·d）（中剂量组）、30μg/（kg·d）（低剂量组），西布曲明灌胃8mg/（kg·d）（阳性对照组），稀释液1.0ml/(kg·d)（肥胖对照组）；模型组和对照组均给予生理盐水1ml/（kg·d)，连续给药10天。末次给药后24h，同时动物禁食过夜后，在水合氯醛麻醉下，测体重、身长，计算LI；断头后取腹部脂肪称重、计算脂肪系数（脂肪重/体重）；腹主动脉取血离心后得血清测总胆固醇（胆固醇氧化酶-过氧化物酶法）、甘油三酯（GPO-PAP法）、高密度脂蛋白（沉淀法）、血糖（GOD-PAP法）和血清胰岛素（放射免疫测定法）水平；断头后取腹部脂肪称重、计算脂肪系数（脂肪重/体重）。结果 ⑴ 造模：正常大鼠（189.40±38.72）g，谷氨酸钠肥胖大鼠平均体重（246.72±36.67）g，（Plt;0.001）；LI分别为289.27±9.05和304.42±9.64（Plt;0.001）。说明造模成功。 ⑵ 药物实验结果：高、中剂量给药组体重、LI、脂肪系数、血总胆固醇、甘油三酯、血糖明显下降达，到统计学要求的显著性；胰岛素水平也有所下降，但未达到统计学要求的显著性（P＞0.05）。结论 rhCNTF 30～300μg/（kg·d)皮下注射连续用药10天，对谷氨酸钠肥胖大鼠具有明显的减肥降脂作用，可能对2型糖尿病肥胖有效。 【关键词】 重组人睫状神经因子；谷氨酸钠肥胖大鼠；降脂作用；降血糖作用 【Abstract】 Objective To investigate the short-term antilipemic effect of recombinant human ciliary neurotrophic factor (rhCNTF) on obesity rats induced by monosodium glutamate (MSG). Methods Obesity models were established by hypodermic injection of MSG to newborn rats. rhCNTF was used at 30～300μg·kg s.c. once a day for 10 days. The body weight and food intake were monitored everyday. After final administration, rats were narcotized with choral hydrate, then the body length and weight were measured to calculate Lee?s index. Blood was sampled through abdominal aorta to assay the serum levels of glucose and lipids. Fat around kidney and genitals and in abdominal cavity was collected for calculation of fat index. Results The body weight and Lee?s index of MSG-rats were significantly higher than those of normal rats, indicating that MSG-Obesity model was established successfully. The short-term administration of high and middle dose of rhCNTF resulted in a significant and dose-dependent reduction of body weight, Lee?s index, fat index, serum triglyceride (TG) and tatal cholesterol (TC), as opposed to profoundly increased high density lipoprotein-cholesterol (HDL-