重组人脑钠肽（rhBNP）对兔血流动力学及电生理影响.docVIP

重组人脑钠肽（rhBNP）对兔血流动力学及电生理影响 　 作者：姚维,杨波, 钱进, 吴钢,陈绪江,余志利【关键词】 重组,遗传；利钠肽,脑；血液动力学过程；动作电位；电生理；兔 　　In vivo cardiac electrophysiological and hemodynamic effects of rhBNP in rabbits 　　【Abstract】 AIM: To observe the effects of shortterm infusion of recombinant human brain natriuretic peptide (rhBNP) on electrophysiologic parameters and hemodynamics in healthy rabbits. METHODS: In 12 healthy rabbits, hemodynamic and electrophysiologic parameters were assessed during infusion of rhBNP at 0.09μg/(kg#12539;min).With monophasic action potential (MAP) recording technique, MAPs of epi, mid and endolayers of myocyte were recorded simultaneously by composite plungeneedle electrode across left ventricular wall. Curve of arterial blood pressure and left intraventricular pressure were recorded through common carotid arterial cannula. RESULTS： Infusion of rhBNP decreased hemodynamic parameters including SAP, DAP, mean arterial pressure(MAP) and LVEDP, LVSP (Plt;0.05)；there were no significant changes in left ventricular pressure ［ (±dp/dt)max］; there were no significant changes in electrophysiologic parameters including MAPs of epi, mid and endo myocardium and surface ECG variable. CONCLUSION: In healthy rabbits, shortterm infusion of rhBNP can change hemodynamic parameters but has no significant effects on electrophysiologic parameters. 　　【Keywords】 recombination, genetic; natriuretic peptide, brain; hemodynamic processes; action potentials; electrophysiology; rabbits 　　【摘要】目的： 观察短期静脉滴注重组人脑钠肽(rhBNP)对家兔的血流动力学和组织电生理作用的影响. 方法： 健康家兔12只，以0.09 μg/(kg#12539;min)静脉滴注rhBNP，应用单相动作电位记录技术，同步记录用药前后心外膜、中层及心内膜心肌单相动作电位(MAP)及体表心电图，分析相关参数的变化；颈总动脉插管，记录动脉血压曲线、左心室内压力曲线变化. 结果： 用药前后，血流动力学指标动脉收缩压(SAP),舒张压(DAP)和平均动脉压(MAP)下降；左室舒张末压(LVEDP),左室收缩压(LVSP)下降(Plt;0.05). 左室内压最大上升、下降速率 (±dp/dt)max用药前后比较无显著差异. 电生理指标3层心肌单相动作电位时程，体表心电图指标无显著差异. 结论： 在健康家兔，短期静脉滴注重组人脑钠肽对电生理指标没有影响，可改变血流动力学指标. 　　【关键词】 重组，遗传；利钠肽，脑；血液动力学过程；动作电位；电生理；兔 　　0引言 　　人脑钠肽（human brain natriuretic peptide, hBNP）属于被人们称为“心脏激素”的利钠肽家族的成员. 利钠肽家族包括心房利钠肽(ANP), BNP, C