Survivin在胆管癌组织中表达及其及VEGF关系相关探究.doc

Survivin在胆管癌组织中表达及其及VEGF关系相关探究 　【摘要】 目的： 探讨凋亡抑制蛋白Survivin在胆管癌组织中的表达及其与血管内皮生长因子(vascular endothelial growth factor，VEGF)表达的关系。方法：应用免疫组织化学方法测定51例胆管癌组织和10例正常胆管组织的Survivin和VEGF的表达水平。结果：1)胆管癌组织中，Survivin阳性表达率为74.5%，正常胆管组织中的阳性表达率为0，两者相比差异有统计学意义(Plt;0.01);Survivin在胆管癌中的表达与患者的性别和年龄、肿瘤的分化程度和部位、有无淋巴结转移无明显相关性(Pgt;0.05)。2)VEGF在胆管癌组织中的阳性表达率为80.4%，在正常胆管组织中的阳性表达率为20%，两者相比差异有统计学意义(Plt;0.01); VEGF在胆管癌中的表达与患者的性别和年龄、肿瘤的分化程度和部位无明显相关性(Pgt;0.05)，而与有无淋巴结转移有相关性(Plt;0.05)，转移组高于无转移组。3)Survivin和VEGF在胆管癌组织中阳性表达的比率均为70.6%，二者的表达密切相关(Plt;0.01)。结论： Survivin和VEGF在胆管癌的发生、发展、侵袭和转移过程中发挥重要作用，二者在肿瘤血管生成过程中密切相关，为胆管癌的诊断和靶向基因治疗提供新的途径。 【关键词】 胆管肿瘤·Survivin·血管内皮生长因子·免疫组织化学 　　【ABSTRACT】 Objective: To investigate the expression of survivin and VEGF in cholangiocarcinoma and explore its relation with clinicobiological behavior. Methods: Immunohistochemical techique was adopted in detecting the survivin and VEGF expression in cholangiocarcinoma and normal bile duct tissues. 51 samples of cholangiocarcinoma were collected in Qilu Hospital of Shandong University from January 2003 to December 2005 , and served as the test group, and 10 samples of normal bile duct tissues as control group. Results: 1) The expression rate of survivin in cholangiocarcinoma was 74.5%, whereas in normal bile duct tissue was 0%, the difference was markablely significant (Plt;0.01). The expression of Survivin in cholangiocarcinoma was not significantly associated with sex, age, tumor differentiation, tumor location and lymph node metastasis (Pgt;0.05). 2) The expression rate of VEGF in cholangiocarcinoma was 80.4%, whereas in normal bile duct tissue was 10%, the difference was significant (Plt;0.01). The expression of VEGF in cholangiocarcinoma was significantly associated with lymph node metastasis (Plt;0.05), but not with sex, age, tumor differentiation, tumor location (Pgt;0.05) . VEGF expression of the patients with lymph node metastasis was higher than that of group without lymph node metastasis. 3) The expression of both survivin and