Ⅲ型前列腺炎前列腺液中IFNγ、IL10、IL4水平变化及临床症.docVIP

Ⅲ型前列腺炎前列腺液中IFNγ、IL10、IL4水平变化及临床症 　 作者：张磊，翁志梁，叶松道，陈映鹤，杨世坤，虞海峰，陈志勇，何有华 【摘要】 目的 探讨Ⅲ型前列腺炎前列腺按摩液中细胞因子IFNγ、IL10、IL4水平变化对临床症状的影响。方法 按照美国国立卫生院(NIH)分类方法诊断Ⅲ型前列腺炎共76例。ⅢA型前列腺炎47例，ⅢB型前列腺炎29例。采用美国国立卫生研究院的慢性前列腺炎症状评分表(NIHCPSI)，评估疼痛症状评分、排尿症状评分；采用双抗体夹心酶联免疫法(ELISA)检测前列腺液(EPS)中IFNγ、IL10、IL4水平，分析各细胞因子变化和症状评分的相关性。结果 ⅢA、ⅢB型前列腺炎两组疼痛症状和排尿症状评分比较无显著性差异(Pgt;0.05)；IFNγ、IL10、IL4均和疼痛症状评分呈正相关(Plt;0.05)，其中IL10起主要相关；IFNγ、IL10、IL4均和排尿症状评分无相关(Pgt;0.05)。结论 Ⅲ型前列腺炎EPS 中IFNγ、IL10、IL4水平和疼痛症状有关，其中IL10可能起主要作用；但IFNγ、IL10、IL4和排尿症状无相关。 【关键词】 前列腺炎；前列腺液；IFNγ；IL4；IL10 ABSTRACT: Objective To discuss IFNγ, IL10, IL4 levels in expressed prostatic secretions(EPS) of type Ⅲ prostatitis and their influence on the symptoms. Methods 76 cases were classified, with type/group ⅢA (47 cases), type/group ⅢB (29 cases) according to NIH classification system. NIH pain symptom index and NIH urination symptom index were compared between the two groups; IFNγ, IL4 and IL10 in EPS were detected by double antibody sandwich enzymelinked immunosorbent assay (ELISA). Results No significant difference of NIH pain symptom index and NIH urination symptom index was detected between the two groups(Pgt;0.05). IFNγ, IL10 and IL4 had positive correlation with NIH pain symptom index (Plt;0.05), and IL10 played the key role. All cytokines had no correlation with NIH urination symptom index (Pgt;0.05). Conclusion IFNγ, IL10 and IL4 in EPS have correlations with pain symptom of type Ⅲ prostatitis, and IL10 probably plays the key role; while IFNγ, IL10 and IL4 in EPS have no correlations with urination symptom. KEY WORDS: prostatitis; expressed prostatic secretions; IFNγ; IL4; IL10 　 Ⅲ型前列腺炎即NIH分类系统炎症性的慢性前列腺炎/慢性骨盆疼痛综合症(chronic prostatitis/ chronic pelvic pain syndrome, CP/CPPS)，是前列腺炎中最常见的类型。越来越多的研究发现，白细胞计数与症状及严重程度之间并无关联[12]，从而促使各国学者积极寻找其他可以造成CP临床症状原因。本文通过对Ⅲ型前列腺炎患者前列腺液(expressed prostatic secretion, EPS)中细胞因子IFNγ、IL10、IL4进行检测，研究其和慢性前列腺炎临床症状的关系。 1 资料与方法 1.1 临床资料 病例均来自2005年4月-2005年10月间温州医学院附属二院泌尿