复方黄芪多糖对环磷酰胺所致小鼠毒副作用影响.docVIP

复方黄芪多糖对环磷酰胺所致小鼠毒副作用影响 　【摘要】 观察不同途径给予复方黄芪多糖对环磷酰胺所致小鼠毒副作用的影响.［方法］通过灌胃及腹腔注射给予小鼠相应剂量的复方黄芪多糖，并行大剂量环磷酰胺腹腔注射，观察小鼠外周血象、免疫器官指数及肝组织丙二醛的含量变化.［结果］复方黄芪多糖腹腔注射组小鼠白细胞及淋巴细胞数明显高于环磷酰胺组，脾脏指数明显升高，肝组织丙二醛含量明显降低.复方黄芪多糖灌胃大剂量组小鼠脾脏指数明显高于环磷酰胺组.［结论］复方黄芪多糖具有拮抗环磷酰胺所致小鼠毒副作用的功能，给药途径以腹腔注射为宜. 【关键词】 黄芪多糖 环磷酰胺 鼠 Effects of the compound astragalus polysaccharides for toxic and sideeffects of cyclophosphamide in mice ABSTRACT:OBJECTIVETo study the effects of compound astragalus polysaccharides (CoASP) administrated in different ways for the toxic and sideeffects of cyclophosphamide (CY) in mice.METHODSMice were randomly divided into the control，CY，CoASP+CY injection group and CoASP by intragastric administration with high dose and low dose. After the given of CoASP，CY was intraperitoneally injected in high does and the peripheral blood hemogram，the immunity organ and the hepatic malondialdehyde (MDA) content changes were observed.RESULTSAs compared with the group of CY, CoAPS injection group significantly increased WBC and lymphocytes and decreased GRA spermatozoa and the hepatic MDA contents, In addition, thymus index was significantly more higher in intragastric administration groups of high dose of CoAPS than in CY group.CONCLUSIONCoASP may have the function of antagonizing the toxic and sideeffects of CY in mice, and the best administration route of the CoASP is intraperitoneal injection. Key words:astragalan;cyclophosphamide;mice 环磷酰胺是临床上常用的广谱抗肿瘤药物，但因它的毒副作用较大，故临床应用受到限制.本研究将黄芪多糖配伍人参总皂苷制备了复方黄芪多糖制剂，探讨了不同途径给予复方黄芪多糖对环磷酰胺所引发的毒副作用的影响. 1 材料与方法 1.1 材料 采用昆明种小鼠，雌雄兼用，体重为18～22g，由延边大学医学部实验动物科提供.膜荚黄芪采集于吉林省和龙市，由延边大学药学院生药学教研室鉴定为膜荚黄芪(Astragalus membraneaceus　Fisch.)的干燥根.环磷酰胺为江苏恒瑞医药股份有限公司产品，丙二醛（MDA）测试盒为南京建成生物工程研究所产品.所用试剂均为分析纯. 1.2 方法 1.2.1复方黄芪多糖注射液的制备 黄芪药材经水提取，乙醇沉淀，透析，得白色粉末状粗多糖(苯酚硫酸法［1］测定多糖质量浓度为832g/kg).人参药材经700mL/L乙醇回流提取，通过D101大孔吸附树脂柱等步骤得黄色人参总皂苷粉末（比色法［2］测定质量浓度为629g/kg）.分别称取提取纯化的黄芪多糖2.0g和人参总皂苷0.4g，加入蒸馏水溶解并定容至200mL，分装，在120℃条件下灭菌30min，冷却，待用. 1.2.2 分组及给药 取60只小鼠，随机分为5个组，即正常对照组、环磷酰胺组、复方黄芪多糖腹腔注射组（10mL/