导痰汤对大鼠非酒精性脂肪性肝炎治疗作用.docVIP

导痰汤对大鼠非酒精性脂肪性肝炎治疗作用 　 作者：应豪 陈汉诚 李凌 周文红 【摘要】 目的 观察导痰汤对实验性大鼠非酒精性脂肪性肝炎（NASH）的影响，并探讨其作用机理。 方法 采用高脂饮食复制大鼠NASH模型，以导痰汤低、中、高剂量分别进行干预，然后观察动物一般情况，计算肝指数，观察肝组织脂肪变性及炎症活动度，并查血清学指标ALT、甘油三脂（TG）、总胆固醇（TCH）、高密度脂蛋白（HCL-C）、低密度脂蛋白（LDL-C）、肿瘤坏死因子-α(TNF-α)，肝组织超氧化物歧化酶（SOD）、丙二醛（MDA）。结果 与空白模型组相比，各剂量治疗组大鼠肝指数明显降低(Plt;0.05)，血清ALT及TG、TCH、LDL- C也有显著降低(Plt;0.01～Plt;0.05)，但HCL-C无明显变化(Pgt;0.05)，能够升高肝组织SOD活性(Plt;0.01～Plt;0.05)，降低MDA含量(Plt;0.05)，血清TNF-α含量显著减少(Plt;0.01)，且表现出一定的量效关系。各治疗组大鼠肝脏炎症活动度也显著改善(Plt;0.05)，但量效关系不明显。 结论 导痰汤有明显防治NASH作用，其机制在于减轻脂类在肝脏的积聚，缓解氧化应激，降低炎症反应，保护肝细胞等作用有关。 【关键词】 非酒精性脂肪性肝炎 大鼠 导痰汤 动物实验 　　【Abstract】 Objective To observe the influence of Daotan decoction on nonalcoholic steatohepatitis(NASH) in rats, and study their relative mechanisms. Methods After NASH in rats was induced by high fat diet, the rats of therapeutic groups were treated with small、middle and high dose Daotan decoction respectively. Then their general condition was observed、 liver index was computed, and fatty degeneration and inflammatory activity of liver were observed . The ALT、triglyceride(TG),total cholesterol（TCH）,low density lipoprotein（HCL-C）,high density lipoprotei（LDL-C）,TNF-α were determined respectively. The SOD ,MDA in liver were determined too. Results The liver index of therapeutic groups were markedly reduced respectively compared with those of model group（Plt;0.05）. ALT, TG, TCH and LDL- C were markedly reduced too (Plt;0.01～Plt;0.05). But HCL-C of therapeutic groups were not significant different from that of model groups（Pgt;0.05）. The activities of SOD in liver were elevated and the content level of MDA in liver was decreased in all therapeutic groups(Plt;0.01～Plt;0.05). the content of TNF-α in serum in all therapeutic groups showed significantly decrease by comparison with model group (Plt;0.01), and there were significant relations of quantity and effect. The inflammation of liver of therapeutic groups were improved better than those in model group too（Plt;0.05）,but there were not significant relat