微波对骨细胞活力及生物力学影响.docVIP

微波对骨细胞活力及生物力学影响 　 作者：李新春 杜靖远 毛宾尧 【摘要】 目的 研究微波辐射对大鼠股骨细胞活力和生物力学的影响，以探求杀灭所有骨、软骨细胞时骨组织生物力学的变化。 方法 32只大鼠处死后，将其双侧股骨完整取出，左侧股骨为实验组，右侧股骨为对照组。应用微波对实验组股骨分别加热3、5、10、15min后，用特殊的乳酸脱氢酶活性染色，判断骨、软骨细胞的活力；用Instron万能材料试验机测定股骨的生物力学并同步记录；用原子吸收分光光度仪测定股骨钙的含量。 结果 实验组与对照组的生物力学及钙含量差异无显著性（Pgt;0.05），股骨经微波加热10min后，温度可达62℃，能够杀灭骨、软骨所有的活细胞。 结论 微波产生的高温，在较短的时间内能杀灭骨、软骨活细胞，而对骨的生物力学无显著损害；通过组织化学观察乳酸脱氢酶活性判断骨、软骨细胞活力的方法是特异和敏感的，体外微波加热可应用于骨肿瘤的保肢手术。 【关键词】 微波 细胞活力 生物力学 骨 乳酸脱氢酶 　　【Abstract】Objective To investigate the effects of microwave induced hyperthermia on cell viability and biomechanics of bone cells in rat’s femur. The biomechanics varieties of rat’s femur after complete eradication of all viable cells in rat bone were studied. Methods 32 rats were euthanized and femurs were removed by disarticulated. The left femurs were heated for 3 minutes, 5 minutes, 10 minutes and 15 minutes with the microwave and the histochemical assay for special lactate dehydrogenase activity was evaluated. The rat bone’s biomechanics and the content of calcium tests were carried out. The right femurs were taken as control. Results There was no significant difference in rat bone biomechanics and the content of calcium between both groups (pgt;0.05) and complete cell death occurred after 10 minutes of heating at 62℃. Conclusion It was found that complete eradication of all viable cells in rats bone could be achieved with minimal reduction mechanical function in shorter time by microwave induced hyperthermia. The method to determine osteocyte’s viability by histochemical assay for lactate dehydrogenase activity proves to be sensitive and specific. Microwave may be applied in limb salvage surgery on patients of bone tumor. 　　【Key words】Microwave Cell viability Biomechanics Bone Lactate dehydrogenase 　　本研究旨在探讨微波加热对骨、软骨细胞活力及骨组织生物力学的影响，最终为临床提供理想的骨肿瘤区段处理方法。 　　1 材料和方法 　　1.1 取材及实验分组 　　32只8周龄健康雄性Wister大鼠（山东大学动物中心提供），体重180～300g，处死后行关节离断，完整取出股骨，仔细分离去除软组织(不损伤骨表面)，自中点用电锯横断一分为二（为使温度计探针能进入髓腔内），用生理盐水冲洗血液及髓内组织后，放入生理盐水中备用。左侧股骨为实验组，右侧股骨为对照组（除不加热外，