微波消融联合瘤体内注射树突状细胞治疗小鼠骨髓瘤实验探究.docVIP

微波消融联合瘤体内注射树突状细胞治疗小鼠骨髓瘤实验探究 　 作者：顾玉明，程隆，周青，许伟，徐浩，祖茂衡 【摘要】 目的 评价微波消融后瘤体内注射树突状细胞对荷瘤小鼠免疫功能的影响以及治疗肿瘤的效果。方法 建立BALB/C小鼠皮下骨髓瘤模型。微波消融肿瘤后瘤体内注射同种异体树突状细胞，24 h后于注射树突状细胞相同位置注射肿瘤坏死因子-α（TNF-α），分别于治疗前1天、治疗后第3、5、7天测量肿瘤体积，第7天流式细胞仪检测外周血T淋巴细胞亚群和肿瘤切片组织病理。与对照组比较免疫功能和肿瘤体积的变化。结果 联合治疗小鼠的外周血CD8+淋巴细胞比例比对照组和消融组增高(Plt;0.05)，并且肿瘤组织病理切片可见T淋巴细胞浸润增加；治疗组肿瘤体积比对照组明显减小(Plt;0.05)。结论 恶性肿瘤经微波热消融后可以暴露肿瘤抗原，联合体外培养的功能完善的树突状细胞可以有效建立肿瘤细胞免疫应答，并有效抑制肿瘤生长。 【关键词】 恶性肿瘤；微波消融；树突状细胞 　　Abstract: Objective To investigate the effect of microwave ablation combined with intratumoral injection of dendritic cells on the immune function of BALB/C mice with myeloma and to evaluate the efficacy in the tumor treatment. Methods BALB/C mouse myeloma model was established subcutaneously, and following microwave ablation, homogeneous variant dendritic cells were injected into the tumor. 24 hours later, tumor necrosis factor-α (TNF-α) was injected in the same site as the dendritic cell injection. The tumor mass was measured one day before and 3, 5 and 7 days after treatment, respectively. On the 7th day, T cell subgroup of peripheral blood was determined by flow cytometry and tumor sections were observed for histopathological characteristics to compare the changes in immune response and tumor size with the control group. Results The proportion of CD8+ lymphocytes in the peripheral blood increased (Plt;0.05) in the combined treatment group as compared to the control group and the ablation group, with evidently increased infiltration in the T lymphocytic tissue; the tumor size in the treatment groups was markedly reduced (Plt;0.05) in contrast to the control group. Conclusion The tumor antigen of malignant tumor can be revealed by microwave thermal ablation. In combination with the intact dendritic cells cultured in vitro, the tumor cellular immunologic response could be established and tumor growth be inhibited effectively. 　　Key words: malignant tumor; microwave ablation; dendritic cells 树突状细胞（dendritic cells，DC）是目前发现的功能最强的专职抗原提