玄参及附子抗鼠心肌肥厚作用对比实验探究.docVIP

玄参及附子抗鼠心肌肥厚作用对比实验探究 　【摘要】 目的：探讨寒性药玄参和热性药附子对大、小鼠心肌肥厚及神经内分泌因子的影响。方法：采用注射异丙肾上腺素及L甲状腺素两种方法分别制备小鼠和大鼠心肌肥厚模型。随机分为5组：正常组、模型组、美托洛尔组（或卡托普利组）、玄参组和附子组。灌胃给药干预7～9 d，称体质量后取血和心脏备用。称心脏质量测定左心室及全心质量指数；放射免疫法测定血浆环磷酸腺苷（cyclic adenosine monophosphate，cAMP）和心肌组织血管紧张素Ⅱ（angiotensinⅡ, AngⅡ）的水平；病理切片HE染色观察心肌细胞横断面面积大小。结果：寒性药玄参能显著改善心脏质量指数，降低cAMP、AngⅡ的含量，明显缩小心肌细胞横断面面积。而热性药附子则无此作用。结论：寒性药玄参能通过抑制心肌肥厚大鼠交感神经系统活性，降低AngⅡ水平而抑制心肌肥厚。而热性药附子抗心肌肥厚及抑制神经内分泌因子分泌的作用不显著。 【关键词】 玄参; 附子; 心肌病, 肥厚性; 对比研究; 大鼠; 小鼠 基金项目: 国家自然科学基金资助项目（No. 　　Gu WL, Chen CX, Wang Y. J Chin Integr Med/Zhong Xi Yi Jie He Xue Bao. 2008; 6(4): 376380. 　　Received September 6, 2007; published online April 15, 2008. 　　Free full text (PDF) is available at . 　　Indexed/abstracted in and full text linkout at PubMed. 　　Forward linking and reference linking via CrossRef.DOI: 10.3736/jcipen Access 　　Comparative study of Scrophulariae and Aconite in inhibiting myocardial hypertrophy in rats and mice 　　Weiliang GU, Changxun CHEN, Ying WANG 　　Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China 　　Objective: To explore the effects of Scrophulariae of cold nature and Aconite of hot nature on myocardial hypertrophy and neuroendocrine factors in rats and mice.Methods: A mouse model of myocardial hypertrophy was established by hypodermic injection of isoproterenol. Sixty myocardial hypertrophy mice were randomly divided into five groups: normal control group, untreated group, metoprololtreated group, Scrophulariaetreated group and Aconitetreated group. A rat model of myocardial hypertrophy was established by peritoneal injection of Lthyroxin. Fifty rats were randomly divided into five groups: normal control group, untreated group, captopriltreated group, Scrophulariaetreated group and Aconitetreated group. After 79 days of treatment with intragastric administration of the corresponding drugs, the effects of Scrophulariae and Aconite on left ventricular weight index (LVWI) and hear