肾阳虚大鼠模型水通道蛋白1改变.docVIP

肾阳虚大鼠模型水通道蛋白1改变 　【摘要】 　　目的：探讨中医学肾阳虚证的物质基础。 　　方法：运用腺嘌呤灌服和氢化可的松肌肉注射2种方法制备大鼠肾阳虚模型（分别称为模型Ⅰ和模型Ⅱ），100只Wistar大鼠随机分为正常对照组，模型Ⅰ组，模型Ⅱ大、中和小剂量组。造模结束后，常规生化法检测血肌酐（serum creatinine，SCr）、血尿素氮（blood urea nitrogen，BUN）、尿肌酐（urine creatinine，UCr）含量，冰点比重法检测尿渗透压（urine osmotic pressure，Uosm）、高岭土法检测尿17羟皮质类固醇含量。实时逆转录聚合酶链式反应检测肾脏组织中水通道蛋白1表达。 　　结果：两种模型大鼠均出现类似肾阳虚证表现。与正常对照组和模型Ⅱ各剂量组比较，模型Ⅰ组大鼠SCr和BUN含量升高（Plt;0.05），UCr含量和Uosm降低（Plt;0.05）。模型Ⅰ组大鼠尿17羟皮质类固醇含量和肾组织AQP1表达比正常对照组下降（Plt;0.05）。与正常对照组比较，模型Ⅱ中剂量组SCr含量增加（Plt;0.05），尿17羟皮质类固醇含量降低（Plt;0.05），而模型Ⅱ各剂量组大鼠肾组织AQP1表达升高（Plt;0.05），并显示出一定的剂量依赖关系，以高剂量组升高最为明显。 　　结论：水通道蛋白可能是肾阳虚证的物质基础之一。 【关键词】 肾阳虚; 水通道蛋白; 大鼠 　　Methods: Two kinds of rat models of deficiency of kidneyyang were induced by adenine intragastric administration (model Ⅰ) and hydrocortisone intramuscular injection (model Ⅱ). One hundred rats were randomly divided into normal control group, model Ⅰ group, lowdose model Ⅱ group, mediumdose model Ⅱ group and highdose model Ⅱ group. After model establishment, contents of serum creatinine (SCr), blood urea nitrogen (BUN), and urine creatinine (UCr) were detected by automatic biochemistry analyzer; freezing point method was used for 24hour urinary osmotic pressure (Uosm) testing and kaolinite method was used to detect the content of urinary 17hydroxycorticosteroid (17OHCS). Expression of aquaporin1 in renal tissue was observed by using real time reverse transcription polymerase chain reaction. 　　Results: The rats of model groups had the characteristics of kidneyyang deficiency syndrome. The contents of SCr and BUN were significantly higher in model Ⅰgroup than in normal control group and three model Ⅱ groups (Plt;0.05), and UCr and Uosm were significantly lower (Plt;0.05). The level of urinary 17OHCS and expression of aquaporin1 in renal tissue were decreased in the model Ⅰ group (Plt;0.05) as compared with the normal control group. Compared with the normal control group, the content of SCr was increased and urinary 17OHCS was decreased in the