膀胱癌患者细胞粘附分子水平临床意义.docVIP

膀胱癌患者细胞粘附分子水平临床意义 　　　【摘要】　目的　探讨细胞间粘附分子(ICAM-1)、血管细胞粘附分子(VCAM-1)与膀胱癌生物学行为的关系。 　　 方法　采用ELISA方法检测26例膀胱癌与10例正常人的血清ICAM-1、VCAM-1。采用免疫组化技术检测ICAM-1在膀胱癌组织和正常膀胱粘膜中的表达。 　　 结果　膀胱癌组术前血清ICAM-1、VCAM-1分别为304.1μg/ml和1 133.6μg/ml，明显高于对照组188.1μg/ml和496.9μg/ml，其含量随肿瘤分级、临床分期递增而升高。膀胱癌组14例术后3个月、6个月随访，血清ICAM-1、VCAM-1较术前明显降低。初发与复发膀胱癌血清ICAM-1、VCAM-1均升高，但两者差异无显著性。膀胱癌组织10例中ICAM-1阳性表达6例。 　　 结论　血清ICAM-1、VCAM-1水平对膀胱癌早期诊断，肿瘤分级，临床分期，预后判断和随访有明确的预示作用。 Serum level of cell adhesion molecule in bladder neoplasms 　　 　　【Abstract】　Objective　To clarify the correlation beween serum intercellular adhesion molecule-1 (ICAM-1)、vascular cell adhesion molecule-1 (VCAM-1) and bladder neoplasm.　Methods　Serum ICAM-1 and VCAM-1 were detected by means of ELISA technique in 26 cases of bladder transitional cell carcinoma.10 cases of nontumor urogenital diseases or normal subjects as controls.　Results　The mean contents of ICAM-1、VCAM-1 were significantly higher in bladder cancer patients (304.1μg/ml、1 133.6μg/ml) than in the controls (188.1μg/ml、496.9μg/ml),These were elevated with the advance of tomor grade and tomor stage.Three and six months after operation,both the ICAM-1 and VCAM-1 were significantlyamp;n bsp;reduced,while in the relapse cases,the ICAM-1 and VCAM-1 would rise again.Immunochemical technique revealed that the rise of serum ICAM-1 content is related to the increased expression of neoplastic tissue.　Conclusions　Serum ICAM-1 and VCAM-1 levels were claimed to be valuable markers in the early diagnosis and prognosis of bladder cancer and is valuable in the follow up study of bladder cancer. 　　【Key words】　Bladder neoplasms　　Carcinoma　　Immunohistochemistry 　　采用ELISA方法测定膀胱癌患者及对照者血清细胞间粘附分子(ICAM-1)、血管细胞粘附分子(VCAM-1)的含量，并采用免疫组化技术测定ICAM-1在正常膀胱移行上皮及膀胱癌组织中的表达，试图了解细胞粘附分子与膀胱癌生物学行为的关系，以探讨其在膀胱癌早期诊断和预后评估中的临床意义。 　　材料与方法 　　一、材料 　　膀胱癌组26例。年龄41～84岁，平均66岁。男21例，女5例。病程15天～2年。初发者18例，复发者8例。病理诊断均为膀胱移行细胞癌。肿瘤分级：Ⅰ级10例，Ⅱ级9例，Ⅲ级7例。临床分期：Ta 12例，T1～T2 9例，T3 5例。于术后3个月及6个月随访14例。术中获得用于本研究的有效膀胱癌组织标本10例。 　　对照组10例。年龄38～76岁，平均63岁。男8