荨麻属植物对骨形成影响探究.docVIP

荨麻属植物对骨形成影响探究 　 作者：史丽颖 阎兴国 周渊 唐玲 冯宝民 王永奇 【摘要】 目的观察4种荨麻属植物的8种不同提取物对骨形成的影响。方法以成骨样细胞MC3T3-E1为靶细胞，以表皮生长因子为阳性对照，4种荨麻属植物的8种不同提取物分为50，100，200 μg/ml 3个浓度组，采用CCK-8 (cell counting kit-8) 活细胞计数法测定4种荨麻属植物的8种不同提取物对成骨样细胞MC3T3-E1的增殖作用，同时测定碱性磷酸酶的活性。结果三角叶荨麻根的醇提物，宽叶荨麻全草的醇提物和醇提物水层过D101大孔树脂40%洗脱部分在50到200 μg/ml 的浓度下能显著促进成骨样细胞MC3T3-E1增殖。同时这3种提取物一定程度上也增强了碱性磷酸酶的活性，但无显著性差异。结论三角叶荨麻和宽叶荨麻提取物在体外对成骨样细胞MC3T3-E1的作用提示其在体内可能促进骨形成过程。 【关键词】 荨麻属植物； 成骨细胞； 增殖； 碱性磷酸酯酶； 活性 Abstract：ObjectiveTo study the effect of eight extracts of four Urtica plants on bone formation. MethodsOsteoblastic MC3T3-E1 cell was used as the target cell, epidermal growth factor was used as positive control, MC3T3-E1 cells were cultured and tested for growth with various concentrations of samples in the medium. The proliferation effect of eight extracts of four Urtica plants on clone MC3T3-E1 cells was examined with cell counting kit-8 (CCK-8) and alkaline phosphatase (ALP) activity was also determined. ResultsWe found that EtOH extract from the root of U. triangularis, EtOH extract and 40% EtOH-H2O fraction with D101 macroporous resin from the H2O partition of EtOH extract from the whole plant of U. laetevirens Maxim. subsp. laetevirens at the concentration of 50 to 200 μg/ml significantly stimulated MC3T3-E1 cell proliferation. The three extracts also somewhat increased ALP activity, but had no significant difference. ConclusionThese effects of EtOH extract from the root of U. triangularis, EtOH extract and 40% EtOH-H2O fraction with D101 macroporous resin from the H2O partition of EtOH extract from the whole plant of U. laetevirens Maxim. subsp. laetevirens on MC3T3-E1 cells in vitro suggest that they may stimulate bone formation in vivo as well. Key words：Urtica Plants; Osteoblast; Proliferation; Alkaline phosphatase; Activity 骨形成和骨吸收是骨代谢的两个基本过程，通常骨形成与骨吸收之间保持平衡，即成骨细胞与破骨细胞保持功能性偶联，但是当某种原因使这种功能性偶联破坏，骨吸收超过骨形成导致骨量减少时即可引起骨质疏松［1，2］。成骨细胞和破骨细胞都是骨代谢过程中的重要核心细胞，而成骨细胞不仅参与骨形成，而且还参与破骨细胞性骨吸收的调节［1，2］。成骨细胞的主要功能有：合成、分泌胶原与糖蛋白，形成骨基质，并通过碱性磷酸酶等多种