凡纳滨对虾(Litopenaeus+vannamei)C-型凝集素免疫作用机制的研究.pdfVIP

Effect of C-type lectin on the immune response mechanism of white shrimp Litopenaeus vannamei Abstract This paper reports the mechanism of immune response mechanism modulated by C-type lectin in the White Shrimp Litopenaeus vannamei, discussed mainly the following theses: (1) Prokaryotic expression of C-type lectin gene of Litopenaeus vannamei; (2) prophenoloxidase （proPO ） activating system and their signal transduction in white shrimp Litopenaeus vannamei haemocytes induced by lipopolysaccharide and recombinant C-type lectin protein; (3) prophenoloxidase （proPO ） activating system and signal transduction of Litopenaeus vannamei induced by lipopolysaccharide and recombinant C-type lectin protein. 1. Prokaryotic Expression of C-type lectin gene of Litopenaeus vannamei and immunologic activity evaluation of Recombinant Protein Litopenaeus vannamei C-type lectin gene (C-type lectin) was expressed in Escherichia coli and the immunologic activity of the recombinant protein was analyzed. Results showed that SDSrevealed the molecular weight of LvLec protein is 39kD, which is consistent with the predicted size. Western blot revealed it could bind with anti-His antibody, which proved that C-type lectin recombinant protein in Escherichia coli succeeded in expressing. For the hemagglutination assay, no obvious agglutination was detected on examined erythrocytes. No obvious agglutination could be observed when Vibrio alginolyticus, Vibrio harueyi, Vibrio anguillarum, Staphylococcus aureus were incubated with LvLec protein. 2. Effect of the recombinant C-type l