芽孢杆菌筛选分离（）.docVIP

芽孢杆菌筛选分离（） Experimental method: Samples: purchased bacteria, commercial microbial preparations (solid or liquid), soil, water or deadwood, rotten leaves (record, sampling location, PH vegetation, etc.). Medium: peptone, 10g/L beef extract, 3-5g/L, NaCl5g/L, PH7.2 Enrichment culture: Take the 5g sediment or gut soluble 0.5g, with 45 (50) mL /250mL (sterile water flask with a glass bead), oscillator 15-20min, 5mL bacterial suspension or 5mL samples adding 45mL bud medium /250mL Erlenmeyer flask, 75 DEG -80 DEG 15-20min water bath to 35 DEG -37 DEG 150-200r/min oscillation 24h. Staining for two t