关节软骨钙化层界线结构特异性染色方法建立.pdfVIP

重庆医学2009 年4 月第38 卷第7 期 799 * 王富友, 杨 柳 , 段小军, 谭洪波, 张 颖, 郭 林, 陈光兴 ( 第三军医大学西南医院关节外科中 , 重庆400038) : 建立关节软骨钙化层界线结构特异性染色方法 从组织库获取成人股骨髁标本10 例, 修剪固定后常 规方法制备石蜡切片, 联合应用 HE天狼猩红番红O/ 固绿和冯库萨等染色方法, 对关节软骨钙化层界线结构进行观察 HE 染色仅能依据各层组织的结构形态区分3 层结构, 两界线显示不清; 天狼猩红染色和番红O/ 固绿染色可以清晰区分粘合 线结构, 但潮线界面显示不清; 冯库萨染色可以清晰显示钙化软骨层两界线结构, 但软骨下骨的形态结构显示不清 通过 对不同方法染色结果进行比较, 番红O/ 固绿染色和冯库萨染色联合应用可以清晰显示钙化层界线结构 : 关节软骨; 钙化软骨层; 石蜡切片; 染色方法 : R681. 3; R446. 8 : A : 167 18348( 2009) * Specific staining method for the limit structure of articular calcified cartilage zone WA N G Fuy ou , YA N G L iu , D UA N X iaoj un , et al . ( Center f or J oint Surgery , Southw e t H o p ital , T hird M ilitary M edical Univ er ity , Chongqing 400038, China) Abstract: Objective T o found the pecific taining metho d for the lim it tructur e of articular calcif ied cartilag e zone. Methods T he normal human co ndyle of femur ( n= 10) wer e donated by the re ident of Chong Qing cit y fro m t i ue bank, and the paraffin ectio n w ere prepared after fixation and decalcificatio n. Ob erv ed the lim it tructure of articular calcif ied cartilage zo ne under the micro cope after tained w ith H E, P icro ir iu , Saf ranin O/ fa t green and vo n ko a. Results T hr eelayer ed tructure of the art icu lar bonecartilag e were di tingui hed according the tructure and hape with H E tain, but the tw o limit tructure could not be ob erv ed clearly. T he cement line w a di tinct w ith t he Picro iriu tain or the Safranin O/ fa t green tain, but the t idemar k could not be ob erv ed clearly . T he tidemar k w a di t inct