氨基甲酸酯类农药残留检测方法对比研究[J].pdfVIP

※分析检测 食品科学 2010, Vol. 31, No. 02 183 郝学飞，董小海，钟红舰 (河南省农业科学院农业质量标准与检测技术研究中心，河南郑州 450002) ：目前蔬菜中氨基甲酸酯类农药残留普遍采用液相色谱柱后衍生法(HPLC)进行测定，研究液相色谱- 质谱 法(LC-MS/MS)测定蔬菜中7 种常用氨基甲酸酯类农药，并与高效液相色谱柱后衍生法进行比较。液相色谱柱后衍 生法是经典方法，重现性好，但是检测周期长，衍生试剂价格昂贵且稳定性差；LC-MS/MS 法保留时间短，灵 敏度高，操作简便，实验过程快，是氨基甲酸酯类农药检测发展的方向。 ：氨基甲酸酯残留；高效液相色谱；液相色谱- 质谱联用仪；比较 A Comparative Study of Post-column Derivatization HPLC and LC-MS/MS Determination of Carbamate Pesticide Residues in Vegetables HAO Xue-fei ，DONG Xiao-hai ，ZHONG Hong-jian (Research Center of Agricultural Quality Standards and Testing Techniques, Henan Academy of Agricultural Sciences, Zhengzhou 450002) Abstract ：In the present study, 7 carbamate pesticides in vegetables were determined using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), and this method was compared with commonly used HPLC with post-column derivatization method. Results showed that the analytes were well separated using both methods, which had good figures of merit. HPLC with post-column derivatization method is considered as a classical method having the advantage of good repeat- ability and the disadvantages of time-consuming operation, expensive derivative reagent cost and poor stability; in contrast, LC- MS/MS, thanks to its short retention time, high sensitivity, simple and easy operation, and rapidity, is more competitive for determination of carbamate pesticides in vegetables. Key words ：carbamate pesticides residues ；HPLC ；LC-MS/MS ；comparison 中图分类号：TQ450.263 文献标识码：A 文章编号：1002-6630(2010)02-0183-04 氨基甲酸酯类杀虫剂对多种害虫有良好的杀虫效 1.1 材料与试剂 果，是最常使用的农药，在农作物栽培过程中，特别