汉黄芩素对肺腺癌A549 细胞黏附和迁移能力的影响及其可能机制[J].pdfVIP

34 肿瘤2011年1月第31卷第1期 Tumor Vol. 31, J anuary, 2011 DOI:10.3781/j .issn.1000-7431.2011.01.007 Basic Research ·基础研究 汉黄芩素对肺腺癌A549细胞黏附和迁移能力的影响及其可能机制 黄恺飞，黄亦琦 厦门市医药研究所药物研发中心，厦门 361003 [摘要]  目的：观察汉黄芩素对人肺腺癌A549细胞黏附、迁移和侵袭能力的影响，并初步探讨其分子作用机制。 方法：采用MTT 法检测汉黄芩素对A549细胞增殖和黏附能力的影响；细胞划痕实验和侵袭小室法检测其对A549 细胞迁移和侵袭能力的影响； RT -PCR和Western 印迹法检测汉黄芩素对A549细胞中与转移相关的基因，如细 胞间黏附分子-1 （intercellular adhesion molecule-1 ，ICAM -1 ）、内皮细胞黏附分子-1 （v ascular cell adhesion molecule-1，VCAM -1 ）和c -Met 表达的影响。结果：汉黄芩素能抑制A549细胞的生长，其细胞增殖抑制率与药 物浓度及作用时间呈依赖关系。低浓度的汉黄芩素处理A549细胞后，可明显降低肺癌细胞的黏附、迁移和侵袭能 力。汉黄芩素能有效抑制ICAM -1、VCAM -1基因和蛋白的表达，但对c -Met 基因及其蛋白并没有影响。结论：汉 黄芩素具有抑制肿瘤细胞转移的作用，其分子机制可能与下调ICAM-1和VCAM-1的表达有关，但与c-Met表达无关。 [关键词]  肺肿瘤；肿瘤转移；细胞黏附分子；汉黄芩素；A549细胞 [中图分类号]  R734.2           [文献标志码]  A               [文章编号]  1000-743 1 （20 11）0 1-0034-05 Effects of wogonin on the adhesion and migration abilities of lung cancer cell line A549 and their mechanisms HUANG Kai-fei, HUANG Y i-qi Drug Research Developmemt Center, X iamen Medicine Research Institute, X iamen 361003，China [A BSTRACT] Objective: To evaluate the effects of wogonin on the adhesion, migration and invasion abilities of A 549 cells, and to explore their molecular mechanisms. Methods: MTT method was used to detect the effect of wogonin on the proliferation of A 549 cells. Scratch test and the invasion chamber model were used to detect the effects of wogonin on the invasion, adhesion, and migration of A 549 cells. T he influences of wogonin on the expressions of intercellular adhesion molecule- 1(ICA M- 1), vascular cell adhesion molecule-1 VCAM-1) and c-Met mRNAs and proteins in A 549 cells were evaluated by RT- ( PCR and Western blotting, respectively . Results: Wogonin was found to inhibit the growth of A 549 cells in dose- and time-dependent manners. Wogonin could significantly red