代谢组学在微生物学中的初步应用.ppt

代谢组学在微生物学中的初步应用 中国科学院大连化学物理研究所1808组 内 容 菌种识别和鉴定 环境和基因改变对菌株代谢的影响分析 抗生素作用机理分析 细菌胞内代谢物提取方法优化 MTG 乳酸乳球菌代谢分析 其他工作 代谢组学技术用于菌种识别和鉴定 自制LPA筛分介质 直接毛细管电泳分析-微米颗粒 直接毛细管电泳分析-纳米颗粒 代谢组学技术用于环境和基因改变对菌株代谢的影响分析 Lp3-3优化 代谢组学技术用于抗生素作用机理分析 抗生素作用差异分析 抗生素 两株菌的代谢物相关分析后的关系 细菌胞内代谢物提取方法优化 代谢淬灭优化 衍生条件优化 MTG 乳酸乳球菌代谢分析 Protein-glutamine γ-glutamyltransferase The gamma-carboxymide groups of peptide-bound glutamine residues act as acyl donors, and the 6-amino-groups of protein- and peptide-bound lysine residues act as acceptors, to give intra- and inter-molecular 6-N-(5-glutamyl)lysine crosslinks. biocyc 脂肪胺类：2-arylethylamine, N-acetyl-2-arylethylamine 氨基酸类：[cytochrome c]-N6-methyl-L-lysine, L-lysine,[cyt c]-L-lysine, N6-acyl-lysine,protein-l-lysine 亮氨酸类似物：N-alpha-acetylornithine, citrulline,D-ornithine, L-ornithine,N5-(L-1-carboxyethyl)-L-ornithine. UPLC-MS name fold tstat pvalue mzmed mzmin mzmax rtmed rtmin rtmax npeaks KO WT 1 M118T122 57.741712 -14.061881 0.0004027033 118.0875 118.0874 118.0878 121.90090 107.80366 131.3408 4 0 4 2 M257T118 181.915483 -13.721746 0.0008313590 257.1467 257.1465 257.1475 117.91472 104.96745 127.1862 4 0 4 3 M258T118 109.403027 -12.589167 0.0009591301 258.1501 258.1499 258.1509 117.71506 104.76487 126.8895 4 0 4 4 M235T126 34.006379 -10.222930 0.0016690655 235.1656 235.1193 235.1662 125.90705 108.51273 143.8020 5 1 4 5 M236T124 Inf -10.182104 0.0020187369 236.1687 236.1682 236.1693 123.58813 108.00625 133.9127 4 0 4 6 M418T531 6.621662 -4.929390 0.0026318292 417.5482 417.5480 417.5507 531.10637 520.51009 544.9151 5 1 4 7 M311T104 1.332506 -5.652667 0.0040897591 311.2188 311.2183 311.2198 104.30771 85.52443 139.0771 8 4 4 8 M132T174 8.499006 -4.998239 0.0042219840 132.1030 132.1029 132.1033 174.19586 159.34896 183.7679 5 1 4 9 M167T99 101.776552 7.790087 0.0043549483 167.0136 167.0135 167.0137 99.1968