沙棘WRI1转录因子基因的生物信息学分析.docVIP

沙棘WRI1转录因子基因的生物信息学分析 　　摘要：以构建的沙棘（Hippophae rhamnoides Linn.）不同组织转录组数据库为基础，分离得到1个沙棘WRI1转录因子编码基因，命名为HrWRI1，利用生物信息学方法对其基因结构、理化性质、保守域、信号肽、亚细胞定位、磷酸化位点和高级结构等进行了预测和较为全面的分析。结果表明，该成员含有1 206 bp的完整开放阅读框，编码401个氨基酸组成的不稳定亲水蛋白，无信号肽和跨膜结构域，存在多个磷酸化位点，定位于细胞核中，高级结构以无规则卷曲为主。与不同植物中已报道的同源WRI1转录因子进行多重序列比对后，发现它们在核酸与氨基酸水平均高度同源，并且具有相同的结构域。 　　关键词：沙棘（Hippophae rhamnoides Linn.）；WRI1基因；生物信息学 　　中图分类号：Q943.2 文献标识码：A 文章编号：0439-8114（2016）22-5972-04 　　DOI：10.14088/ki.issn0439-8114.2016.22.061 　　Bioinformatics Analysis of WRI1 Gene in Hippophae rhamnoides 　　MA Qian，LI Jing-bin，RUAN Cheng-jiang，LI Rong-rong 　　（Resources and Plant Research Institute/College of Environment and Resources，Dalian Nationalities University，Dalian 116600，Liaoning，China） 　　Abstract： In the present study，a HrWRI1 gene firstly based on the transcriptome sequencing data from H. rhamnoides was obtained. Then，cDNA and deduced amino acid sequence， physical and chemical characterization，conserved domain，signal peptide，subcellular localization，phosphorylation site molecular modeling were predicted and analyzed. It follows that this predicted cDNA has an open reading frame of 1 206 bp in length，encoding protein of 401 amino acids. It was hydrophilic proteins，no transmembrane regions and signal peptide，contain multiple phosphorylation sites. A subcellular localization analysis predicted that they may exist in the nucleus. In addition，the secondary structure is mainly based on random coil. In comparison to the other known WRI1 from various species，the overall sequence alignment suggested that they were highly similar at the protein level，especially conserved domain. Our investigation could definitely provide a significant foundation for further research on function analysis of HrWRI1. 　　Key words： Hippophae rhamnoides Linn.； WRI1 gene； bioinformatics 　　沙棘?伲?Hippophae）系林奈于1753年以沙棘（Hippophae rhamnoides Linn.）为模式建立的。沙棘又名醋柳、酸刺等，为胡颓子科沙棘属落叶灌木或小乔木，是一种新兴的小浆果类树种[1，2]。近年来，植物油脂的需求量越来越多，利用基因工程技术提高油料作物中植物油的含量已经成为最有发展前景的方法之一，培育高含油量的沙棘品种已成为沙棘研