落叶松扦插生根过程抑制消减杂交文库构建及分析-林业科学研究.pdfVIP

2009, 22( 5): 672~ 676 F orest R esearch : 2009) 1, 2 1 1 3 1* 冯 健 , 齐力旺, 孙晓梅, 韩素英 , 张守攻 ( 1. , 100091; 2. , 110032; 3. , 100091) : 2, 2 cDNA , : 2 cDNA PCR , , , 521 Un Ei ST : ; ; : S791. 22 : A Construction and Analysis of the Suppression Subtractive Hybridization L ibrary ofLarix Cuttings during Rooting 1, 2 1 1 3 1 FEN G J ian , QI L iwang , SUN X iaom ei, HAN Suy ing , ZHANG Shougong ( 1. Group of Trees C ell B iology, Research Institute of Forestry, CAF, B eijing 100091, Ch ina; 2. Liaon ing A cademy of Forestry, Shenyang 110032, L iaon ing, Ch ina; 3. Research Institute o f Forest Eco logy, E nvironm ent and Protection, CAF, Beijing 100091, Ch ina) Abstract: T aking the stem s and roots of wt o fu llsib clones w ith significant d ifference in rooting rate as test m aterials, tw o differential expression cDNA librar ies of Lari cutting during rooting w ere established by PCR TM select cDNA subtraction k it. The resu lts of analysis of Rsa I d igestion, ligation and subtraction eff iciency show ed that the tw o libraries w ere established successfully. The positive clones w ere screened by PCR and dot blots. 521 UniEST w ere obtained by sequenc ing and b io inform atics analysis. K ey words:Lari ; suppression subtractive hybrid ization library; cutting (Lari spp. ), ; [ 1- 5] , , , , , ( Suppression Subtractive H ybridization, , ,