异氟烷对老年大鼠认知功能及海马nmda受体nr第三军医大学学报.docVIP

异氟烷对老年大鼠认知功能及NMDA受体NR1亚基磷酸化的影响 张宏金1 丁月东1 蔡虹 2 徐敏3 陈小兵4 张晓群1 摘要：目的 探讨老年大鼠吸入异氟烷后其认知功能变化及NMDA受体NR1亚基磷酸化的分析。方法 应用18月龄SD大鼠24只，雌雄各半，随机分成3组：0%异氟烷组(I0%)、1.2%异氟烷组（I1.2%）和1.8%异氟烷组（I1.8%），每组8只。每只老鼠分别放置在特制的连接麻醉机及气体监侧仪麻醉箱，按上述分组处理分别连续吸入5天，每天60min。自第6天始连续5天对各组大鼠行Morris水迷宫测试，以定位航行实验中到达平台的潜伏期作为学习成绩，以空间探索实验中在1min内通过平台位置的次数作为记忆成绩。结束后断头取大鼠海马，应用westtern-blot法检测NMDA受体NR1亚基磷酸化的水平。 结果 (一) 水迷宫检测 (1)三组均随着游泳天数增加定位航行实验潜伏期缩短（与同组第一天比较P0.01）；(2) I1.2%组与I0%组在第1天潜伏期比较有显著性差异（P0.05）第2天以后各时间点潜伏期和空间探索实验的成绩与I0%组同时间比较均无差异（P0.05）；(3) I1.8%组与I0%组潜伏期和空间探索实验的成绩比较均有显著性差异（P0.05）。（二）westtern-blot法检测NMDA受体磷酸化NR1分析 (1) I1.2%组与I0%组NMDA受体磷酸化NR1分析比较无显著差异(P0.05)。(2) I1.8%组与I0%组NMDA受体磷酸化NR1分析比较有显著性差异(P0.05)。 结论 (1)老年大鼠连续5天，每天吸入1.2%异氟烷60min，对早期空间学习能力短暂影响，对其后及空间记忆无明显影响。对海马NMDA磷酸化NR1表达无明显影响。(2) 老年大鼠连续5天，每天吸入1.8%异氟烷60min，对空间学习记忆及能力有明显影响，并且能减少海马NMDA磷酸化NR1的表达。推测1.8%异氟烷影响老年大鼠的认知功能可能与减少海马NMDA 受体NR1亚基磷酸化有关。 关键词：异氟烷；磷酸化；NR1亚基；NMDA受体；认知功能 【中图分类号】R395．1 【文献标识码】A 【文章编号】 ffects of isoflurane on cognitive function and phosphorylation of NMDA receptor 1 subunits on hippocampus in aged rats Zhang Hong-jing1，-Cheue-dong1， Cai-Hong2,et al.(1. Department of Anesthesiology; Lianyungang Second People’s Hospital;Lianyungang 222023;P.R.China;2. Department of Surgery; Lianyungang Second People’s Hospital ;Lianyungang 222023;P.R.China; 3.Department of Oncology; Lianyungang Second People’s Hospital ;Lianyungang 222023;P.R.China) ABSTRACT: Objective To study the effects of the different level of isoflurane on the cognitive function and phosphorylation of NMDA receptor 1 subunits on hippocampus in aged rats． Methodsof either sex were enrolled. They were randomly divided into 3 groups ( n=8/group)：the normal control group (I0% group)、I1.2% group inhaled 1.2% isofluranne; I1.8% group inhaled 1.8% isofluranne. A11 rats received 1.2％ or 1.8％ isoflurane in a special ass anesthesia box for 60 mintures every day for 5 consecutive days respectively except the normal control．The Morris water maze testing was performed every day fo