层层自组装法制备百里香精油微胶囊条件优化-食品科学.pdfVIP

※工艺技术 食品科学 2016, Vol.37, No.02 51 层层自组装法制备百里香精油微胶囊条件优化 黄 晶，申莉莉，白友菊，马治灿，陈加平，冯 武* （华中农业大学食品科学技术学院，湖北武汉 430070 ） 摘  要：采用层层自组装的方法，以百里香精油乳化液纳米微粒作为核心，利用壳聚糖和海藻酸钠对百里香精油 进行包埋；以司盘80和吐温60复配乳化剂亲水亲油平衡（hydrophilic lipophilicbalance  ，HLB ）值、用量及油乳比 例3 个因素进行实验，确定乳化液最适制备条件；以壁材溶液质量浓度、组装层数、每层组装时间、搅拌速率、 组装温度、壁材溶液pH值和固化剂用量进行单因素试验，以主要因素设计正交试验，确定最佳包埋条件。结果表 明，乳化液制备条件为HLB值10、乳化剂用量6%、精油与乳化剂体积比1∶6，此条件下所制备的乳化微粒为模板， 组装层数4 层、搅拌速率1 040 r/min、每层组装时间15 min、壁材溶液pH 5.0 、组装温度30 ℃，此条件下包埋率最 高为80.23%。 关键词：层层自组装；微胶囊；百里香精油；正交试验；包埋率 Condition Optimization for Thyme Oil Microcapsules Prepared by Layer-by-Layer Self-Assembly Method * HUANG Jing, SHEN Lili, BAI Youju, MA Zhican, CHEN Jiaping, FENG Wu (College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China) Abstract: In this work, using thyme oil emulsion nanoparticles as the core, chitosan (CS) and sodium alginate (ALG) were employed for embedding thyme essential oil by a layer-by-layer self-assembly method. Three emulsification conditions including HLB (hydrophilic lipophilicbalance)   value of 1:1 mixture of Span 80 and Tween 60, emulsifier concentration and ratio of oil to emulsifier were optimized to be 10, 6% and 1:6, respectively. The maximum microencapsulation efficiency of 80.23% was obtained when self-assembly of the emulsion particles in four layers by alternate use of CS and ALG was conducted for 15 min at 30 ℃ with stirring at a speed of 1 040 r/min, as determined by orthogonal array design. Key words: self-assembly; microcapsule; thyme oil; orthogonal array design; embedding rate DOI:10.7506/spkx1002-6630-201602009 中图分类号：TS202.3