In recent years, the effect of micro - mechanical environment on the biological
behaviors of cells attracted widespread attention. A variety of cell biological behaviors,
such as cell morphological adjustment, migration orientation changes, proliferation and
differentiation changes, were influenced by rigidity of adhesion substrate for most tissue
cells which were anchorage dependent in vivo. These biological behaviors were closely
related to many physiological and pathological processes. Biochemical environment and
biophysical environment were two key problems needed to be solved in constructing a
cell culture model in vitro to simulate the cell growth environment. The former part had
been gradually improved by biologists and medical scientists, and substrate rigidity was
most notably of effects of biophysical factors. Mathematical simulation and quantitative
control of substrate for cell culture in vitro would be the core of this work.
Hydrogels are water - soluble polymers which have the very similar water content
to macromolecular compounds in vivo, they have been widely used as biomedical
materials as their better biocompatibility. A polyvinylalcohol (PVA) hydrogel which
used as substrate material for cell culture in vitro to study respond rules of cell to
mechanical environment was prepared by chemical cross - linking method.
Glutaraldehyde was used in this study as the crosslinker to prepare saturated PVA
hydrogel which improved the mechanical strength and thermal stability of the film, and
a small amount of glutaraldehyde had the function of sterilization and disinfection.