络合萃取分光光度法测定烟草提取液中的烟碱 determination of nicotine in aqueous tobacco extract by complex-extracting-spectrophotometry.pdfVIP

络合萃取分光光度法测定烟草提取液中的烟碱 吴苏喜1一，董君英1 1长沙理I大学，长沙市天G区赤岭路45号410076 2．湖南农业大学，长沙市芙蓉区马坡岭410028 关键词：烟碱；分光光度法；烟草提取物；络合；萃取 摘要：利用烟碱与甲基橙的络合物在氯仿中的吸光度与其浓度呈正比的原理，建立了测定烟草 提取液中烟碱含量的络合萃取分光光度法，即在20mL烟草提取液中依次加入2．0mL(pH5．0) 柠檬酸／磷酸氢=钠缓冲溶液和2．0mL甲基橙饱和溶液，摇匀，加入20mL氯仿、于室温下振荡 萃取2～3rain，静置3～5rain，取氯仿萃取液于6min内测定其在420nm波长处的吸光度，然后根 据工作曲线计算其烟碱含量。该法的线性相关范围是0．0425～0．2550 mg／mI．．相关系数为 ★烟草科技_roha^8∞ci。胃。却Te}—S★ 0．9994，平均回收率为98．32％，CV5％。 中图分类号：TS41I．1文献标识码：B文章编号：1002—0861(2006)02—0033—04 DeterminationofNicotinein TobaccoExtract Aqueous by WU DONG SU-XI(1，2)andJUN—YING(1) ofScienceand 410076，China 1．ChangshaUniversity Technology，Changsha 2．Hunan 410028，China AgriculturalUniversity，Changsha Keywords：Nicotine；Spectrophotometry；Tobaccoextract；Complex；Extraction Abstract：Amethodof determinationofnicotineinan complex··extracting—spectrophotometrlc aqueous tobaccoextractwas virtueofthe thatthe ofa ofnicotine developedby principle lightabsorptivitycomplex and isin toitsnicotineconcentrationwhilethe isinchloroform．In methyl-orangeproportion complex this mLtobaccoextractwasreactedwith2．0mL method，2．0 saturated solutioninthe methyl．orange of2．0 acid／sedium buffer presence mL(pH5．0)citrichydrogenphosphatesolution。extractedwith ★烟草化学T0bacn口nlI。萤暑七 20mLchloroformatlOOm for2 to3 for3to5 thendetermined temperatureminutes，stoodminutes，and the at the am of420 within6minutes．’11lecontentofnicotinewas lightabsorptivitywavelength with calculatedthecalibrationcurveThe ofthe