番茄颈腐根腐病病原鉴定及不同条件对其生长的影响-植物病理学报.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA　 42(5):449-455(2012) 番茄颈腐根腐病病原鉴定及不同条件对其生长的影响 1 1 2 2 1* 耿丽华 , 李常保 , 迟胜起 , 王丽君 , 柴 敏 1 2 ( 北京市农林科学院蔬菜研究中心, 北京 100097; 青岛农业大学农学与植保学院, 青岛266109) 摘要:作者于2007年在北京郊区大棚内发现番茄(Lycopersicon esculentum Mill.)颈腐根腐病。 为了促进我国开展番茄颈 腐根腐病的防治研究和抗病品种培育工作,有效控制番茄颈腐根腐病的危害,采集表现典型症状的发病植株进行病原分 离、纯化,结合传统的形态学方法和rDNA-ITS序列分析方法,并进行了该病原菌的适宜发病土壤温度测定和致病范围试 验,在此基础之上对病原菌进行了鉴定。 本论文还对该病原菌生长的影响条件进行了研究,确定该病由尖孢镰刀菌番茄颈 腐根腐专化型(Fusarium oxysporumf.sp. radicis-lycopersici)侵染所致;该病原菌在PDA培养基上生长适宜温度为25℃,最 适pH为8;其较适宜的氮源是有机氮和硝态氮,铵态氮不适宜其生长;菌丝致死温度为63℃、10 min;分生孢子萌发的最适 相对湿度是100%,最适温度为25℃,分生孢子致死温度为61℃、10min。 这是首次关于尖孢镰刀菌番茄颈腐根腐专化型导 致番茄病害在我国发生和研究的报道。 关键词:番茄;颈腐根腐病;病原;鉴定;影响条件 Identificationof the pathogen causing Fusarium crown and root rot of tomato and its growth affecting factors 1 1 2 2 　 GENG Li-hua , LI Chang-bao , CHI Sheng-qi , WANG Li-jun , CHAI Min 　1 1 (Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097, China; College of Agronomy and Plant Protection,Qingdao Agricultural University,Qingdao266109,China)2 Abstract: Crown androot rot disease of tomato(Lycopersicon esculentum Mill.)wasfirst observed in green- houses inBeijingsuburbin2007. Inordertolaythebasisforresistancebreedingandfindcontrollingmeasures for the disease,the typical diseased plants were collected and the pathogen was isolated and identified accor- ding to the morphology,the sequence of internal transcribed spacer region of the ribosomal DNA (ITS),the host rangealongwiththeoptimal soiltemperatureatwhichthediseaseoccurred. Themajoraffectingfactorsof the pathogen growth were also studied. The resultswere showed as follows:the pathogen of the disease was