【精选】猪繁殖与呼吸综合征病毒S-1株ORF5和ORF7基因的克隆及变异分析.pdfVIP

2008, 24( 2): 40- 45 Acta Agriculturae Shanghai : 1000-3924( 2008) 02-40-06 S-1ORF5ORF7 1 1 2 1 1 1* 1 1 张春玲 , 张婉华 , 臧克伟 , 王 英 , 蒋凤英 , 邹 勇 , 李春华 , 何锡忠 1 2 ( , 201106; , 271018) ) : PRRSV S-1 M arc-145, RT-PCR ORF5 ORF7 2 PET-32C, , : PRRSV S-1 ORF5 , VR-2332 CH-1a: 91% 89% , 87% 85% ; ORF7, 97% 94% , 95% 93% : ; ORF5; ORF7; : S828; S855 : A Clone and variation analysis of the ORF5 and ORF7 genes of Porcine Reproductive and Respiratory Syndrom e Virus (PRRSV) S- strain 1 1 2 1 ZHANG Chu -li g , ZHANG W a -hua, ZANG Ke-w ei, WANG Y i g, 1 1* 1 1 JIANG Fe g-yi g, ZOU Yo g , LIChu-hua, HE X-i zho g 1 ( AnimalH usbandry and Veterinary R esearch Institute, ShanghaiA cademy of Agricultural Sciences, 2 Shanghai 201106, China; College of Veterinary M ed icine, Shandong Agricultural University, aian 271018, Ch ina) Abstract: A fter the revival of PRRSV S-1 strai byM arc-145 cells, theORF5 a d ORF7 ge es of PRRSV S-1 strai w ere amplified by RT-PCR a d clo ed d irectly i to the prokaryotic expressio vector pET-32. The se- que ce a alysis show ed that the variability of ORF5 ge e w as relatively h igh a d the homology betw ee the PRRSV S-1 a d VR-2332 strai s a d bewt ee the PRRSV S-1 a d CH-1a strai s w as respectively 91% a d 89% i ucleotide a d 87% a d 85% i am i o acid. The ORF7 ge e w as relatively co servative,