大强度耐力训练和不同剂量补铁对大鼠血清铁、血红蛋白及骨骼肌线粒体呼吸链功能的影响.docxVIP

生垦垩塑堕兰壅查兰Q!!竺竺旦笙丝鲞笙!塑垦旦垫!璺P!坐丛鲤!!!堕：!Q!!!!!!：!!!堂：竺·793·大强度耐力训练和不同剂量补铁对大鼠血清铁、血红蛋白 及骨骼肌线粒体呼吸链功能的影响李洁王博 西北师范大学体育学院(兰州730070)摘要目的：探讨大强度耐力训练和不同剂量补铁对大鼠血清铁、血红蛋白及骨骼肌线粒体呼 吸链功能的影响。方法：40只雄·睦Wistar大鼠随机分为5组(扎=8)：对照组(C组)、训练组(T组)、小剂 量补铁+训练组(S组)、中剂量补铁+训练组(M组)和大剂量补铁+训练组(L组)。训练大鼠进行每周6 天、共8周的递增负荷运动。补铁组大鼠第5～8周补铁，小剂量为28．85 mg／d／kg，中剂量为57．7 mg／d／kg，大剂量为115．4 mg／d／kg。训练结束后取血液及骨骼肌样本，测定血红蛋白(Hb)、血清铁含量及骨骼肌线粒体呼吸链酶复合体I～Ⅳ(C I～Ⅳ)活性。结果：1)与C组相比，T组血清铁含量显著下降(P0．01)，M组和L组血清铁含量均显著升高(P0．05或P0．01)。与T组相比，S组、M组和L组血清铁 含量均显著升高(P0．01)。2)与C组相比，T!lIHb含量显著下降(P0．01)。与T组相比，S组、M组和 L组Hb含量均显著升高(P0．01)。3)与C组相比，T组和S组C I～Ⅲ活性均显著升高(P0．01)，CⅣ 活性显著下降(尸0．01)；M组C I～Ⅳ活性均显著升高(P0．01)；L组C I和CⅢ活性显著升高(P 0．01)，CIV活性显著下降(P0．01)。与T组相比，S组C I、CⅡ和CⅣ活性均显著下降(P0．01)．CⅢ 活性显著升高(P0．01)；M组C I。Ⅳ活性均显著提高(P0．01)；L组C I、CⅡ和CⅣ活性均显著下 降(P0。01)。结论：大强度耐力训练引起大鼠缺铁性贫血，司ll练期阊补铁可改善贫血状况，中剂量 补铁提高骨骼肌线粒体呼吸链功能的效果最佳。关键词耐力训练；补铁；大鼠骨骼肌；呼吸链酶活性Effects of Training and Iron Supplement on Serum Iron，Hemoglobin and Functionof Mitochondrial Respiratory Chain in Skeletal Muscle of Rats￡i J／e．WangBoco讹伊ofPhysical Education，Northwest Normal踟幻e瑚毋，Lanzhou，China 730070 Corresponding A uthor：Li五e，Email：lijie2005ty@126．comAbstract Objective The aim of present study was to investigate the effects of exe]cise and iron supplement Off mitochondrial respiratory chain in skeletal muscle of rats．Methods Forty healthy male Wistar rats weFe equally randomized to 5 groups：sedentary control(C)，training(T)，training plus supplementation of small dose iron(S)，training plus supplementation of medium dose iron(M)and training plus supplementation of large dose iron(L)．1墨ats in aU training groups performed incremental exercise 6 days per week for 8 weeks．Iron supplementation was applied to rats from fifth to eighth week． 28．85 mg／cVkg，57．7 mg／d／kg，and 1 15．4 mg／d／kg of ferrous sulfate were supplied to groups T，S，M，and L，respectively．After experiment，rats were sacrificed and blood and skeletal muscle samples were obtained．Hemo斟obin(Hb)，serum iron and activities of