爪蟾基因组.pdf

Genetica 108: 163–169, 2000. 163 © 2000 Kluwer Academic Publishers. Printed in the Netherlands. Glider and Vision: two new families of miniature inverted-repeat transposable elements in Xenopus laevis genome 1 ´ 2 2 ´ ´2 ´ 2 David Lepetit , Stephanie Pasquet , Michelle Olive , Nadine Theze Pierre Thiebaud 1UMR CNRS 5558, Universit´e Lyon I, 69622 Villeurbanne, France; 2 Unit´e INSERM 441, Universit´e Bordeaux 2, 33600 Pessac, France; Author for correspondence (Phone: 33 5 57 89 19 76; Fax: 33 5 56 36 89 79; E-mail: pierre.thiebaud@bordeaux.inserm.fr) Received 18 April 2000 Accepted 23 June 2000 Key words: repetitive DNA, transposable element, Xenopus laevis Abstract We have characterised from Xenopus laevis two new short interspersed repetitive elements, we have named Glider and Vision, that belong to the family of miniature inverted-repeat transposable elements (MITEs). Glider was first characterised in an intronic region of the -tropomyosin (-TM) gene and database search has revealed the presence of this element in 10 other Xenopus laevis genes. Glider elements are about 150 bp long and for some of them, their terminal inverted repeats are flanked by potential target-site duplications. Evidence for the mobility of Glider element has been provided by the presence/absence of one element at corresponding location in duplicated -TM genes. Vision element has been identified in the promoter region of the cyclin dependant kinase 2 gene (cdk2) where it is boxed in a Glider element. Vision is 284 bp long and is framed by 14-bp terminal inverted repeats that are flanked by 7-bp direct repeats. We have estimated that there are about 20,000 and 300 copies of Glider and Vision respectively scattered througho