引物退火控制技术在差异表达基因克隆中的应用Application-海洋科学.PDFVIP

Application of annealing control primer system to cloning of differentially expressed genes 1. 361021 2. Q781A1000-3096200705-0070-06 cDNA 1992 Liang [1] DDRT-PCR mRNA (Annealling Control (mRNA Differential Display Reverse Transcription [12] Primer, ACP PCR, DDRT-PCR) 1 ACP (Representational Difference Analysis, 13 RDA) RNA ( RNA Arbitrarily Primed 53 PCR,RAP-PCR) RC4D(RFLP-coupled Differential 5 Display)SAGE(Serial Analysis of Gene Expression) Suppression Subtractive [2] cDNA-AFLP iAFLP [poly(dI)] 5 (dI) Hybridization, SSH) [1314]ACP (Introduced Amplified Fragment Length Polymorphism) ( DNA Chip Technique) PCR 2 1.1 RNA mRNA dT-ACP1 [38] cDNA PCR 2006-11-13 2007-03-03 2005N041(205079) (JA004229) 1976-