碘甲烷对雄性大鼠肝脏氧化损伤的研究-中国试验动物学报.DOCVIP

碘甲烷对雄性大鼠肝脏氧化损伤的研究 马晓明，宋祥福，张 迪，张 晶，侯雨霏，李铁骥* （吉林大学公共卫生学院，长春 130021）研究碘甲烷对雄性大鼠肝脏的氧化损伤作用。方法 将24只雄性SD大鼠随机分成4组，实验组分别给予、的碘甲烷，每天染毒4小时，染毒一周，对照组给予。对血清、肝脏组织中超氧化物歧化酶（SOD）和血清中谷胱甘肽S转移酶（GST）的活力测定。结果 大鼠血清、肝脏组织中SOD活力结论 碘甲烷暴露对大鼠的肝脏氧化损伤作用。超氧化物歧化酶谷胱甘肽S转移酶Study on liver oxidative damage in male rats with methyl iodide MA Xiao-ming，ZHANG Di，ZHANG Jing，LI Tie-ji （School of Public Health of Jilin University，Changchun，130021，China） 【Abstract】 Objective To explore the oxidative damage effect of methyl iodide on the liver of male rats. Methods Twenty-four male SD rats were randomly divided into 4 groups, put them into the static exposure tank. Three experimental groups were given the methyl iodide with concentrations of 650mg/m3, 260mg/m3and130mg/m3 respectively for a week period of time. The duration of daily inhalation was 4 hours, The control group was given the same environmental conditions without exposure. At the end of the exposure，the level of superoxide dismutase (SOD) in serum and liver tissue and the activity of glutathione S transferase (GST) in serum were measured. Results With the increase of methyl iodide concentration, the SOD activity in the serum and liver tissue of rats decreased. There was significant difference, between experimental groups and control group. Serum GST activity gradually increased with the increase of the concentration of methyl iodide. There was significant difference between the experimental group and the control group, and there was also significant difference among the low dose group, middle dose group and high dose group in the serum level of GST. Conclusions Methyl iodide exposure may has the effect of oxidative damage on rat liver. 【Key words】 methyl iodide；SOD；GST；liver；oxidative damage 碘甲烷是工业上一种常用的甲基化试剂，受热易分解，产生有毒的碘化物烟气，可经呼吸道、皮肤、消化道吸收而引起中毒[1]。国内外曾报道多例碘甲烷中毒的病例，症状主要包括：头晕、意识模糊、恶心、呕吐、皮肤损害等。国外的研究提示碘甲烷对实验动物主要的影响包括：神经系统损害、鼻粘膜细胞损害、以及潜在的致癌性[2-8]，而对碘甲烷的肝脏毒性研究较少，并且碘甲烷和绝大多数有机溶剂一样，进入机体后主要通过肝脏进行代谢。因此为了解碘甲烷对肝脏的氧化损伤作用，故进行本次研究。由于本次报道是碘甲烷毒性研究实验的一个分支实验，还需要进一步研究生殖