卡托普利对急性肺损伤mpo和tnf-α的影响.docVIP

卡托普利对急性肺损伤MPO和TNF-α的影响 韩鹏凯，张婷，王导新（重庆医科大学附属第二医院呼吸内科，重庆 400010） 摘要 目的 作者研究急性肺损伤(ALI)时髓过氧化物酶(MPO)和肿瘤坏死因子-α(TNF-α)（哪里的？）的变化及卡托普利对其的影响。方法 将30只SD大鼠随机分成3组：生理盐水对照组、脂多糖(LPS)组（5mg/kg）及其卡托普利治疗组（1.25mg/kg），每组各10只。（用药时间？损伤后多长时间用？用后多久取组织测量？有无时间分组？）观察各组大鼠动脉血氧分压（PaO2）、肺湿质量/干质量值（W/D）、支气管肺泡灌洗液(BALF)蛋白含量及肺组织病理变化，检测肺组织中髓过氧化物酶活性，血浆和支气管肺泡灌洗液TNF-α水平，数据利用SAS 9.1统计软件进行分析。（什么软件不重要，重要的是采用什么方法）结果 脂多糖处理后大鼠的肺部炎症反应显著，以中性粒细胞浸润为主，PaO2下降、肺W/D升高、BALF蛋白含量增加，肺组织MPO活性及血浆和BALF中TNF-α含量均升高（P0.05）。与脂多糖组相比，卡托普利治疗组，PaO2和肺组织病理变化明显改善，肺W/D、BALF蛋白含量、肺组织MPO活性及血浆和BALF中TNF-α含量显著降低(P0.05)。结论 卡托普利对急性LPS介导性致急性肺损伤具有一定的保护作用。 关键词：急性肺损伤；卡托普利；髓过氧化物酶；肿瘤坏死因子-α 中图法分类号：R322.35；R329.27；R392.11 文献标识码：A Effect of captopril on MPO and TNF-α in acute lung injury HAN Peng-kai，ZHANG Ting(Department of respiratory disease, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010，China)Abstract: Objective To study the change of myeloperoxidase (MPO) and tumor necrosis factor-α (TNF-α) in acute lung injury and the effect of captopril. Methods Sprague-Dawley rats were randomly divided into 3 groups, a saline control group, a lipopolysaccharide (LPS) groups (5mg/kg), and a LPS+captopril group(1.25mg/kg), each group 10 rats. The level of PaO2, wet/dry ratio(W/D), the concentration of protein in bronchoalveolar lavage fluid (BALF) and lung tissue histopathological changes were examined, the activity of MPO in lung and the level of TNF-α in serum and BALF were measured. The datas were analysed by SAS 9.1 software. Results Histological examination showed that extensive lung inflammation were seen in the LPS group, which manifested by accumulation of significant numbers of neutrophils. The level of PaO2 decreased and the level of W/D, concentration of protein in BALF were increased significantly, together the activity of MPO in lung and the level of TNF-α in serum and BALF ascended (P0.05). Compared with LPS group these changes were markedly attenuated in captopril group (P0.05)，together the level of PaO2 and pathological ch