区分两个中国李品种的微卫星标记的开发.pdfVIP

区分两个中国李品种的微卫星标记的开发 薛华柏1’2，乔玉山1，章镇卜，曹尚银2，郭俊英2，张慧蓉3 (1南京农业人学同艺学院，江苏南京210095；2中国农业科学院郑州果树研究所，河南郑州450009；3河南科技学院， 河南新乡453003) 摘要：利朋具有高多态信息含量，基于限制性内切酶和简单重复序列的分子标记技术筛选两个 中国李品种问的差异，电泳后从聚丙烯酰胺凝胶上回收差异片段，测序后设计一条引物与微卫星引物 配对使用，即可将其转化为微卫星特异标记(当然也是SCAR标记)。结果从小黄李中分离的一条与盖 县人李的筹异片段，被成功转化为区分这两个品种的特异微一l蔓星标记。 关键词：李；微卫星；SSR；分子标记 Prunus ofaMicrosatelliteMarker TwoVarietiesof Development among salicinaLindl． XUE ZHANG Hua-bo他，QIAOYu-Shanl，ZHANGZhenh，CAO Shang-Yin2，GUOJun-Yin92，and Hui—Ron93 2 Fruit 1 (College。ofHorticulture,NanjingAgriculturalUniversity；Nanjing Research Institute Institute,Chinese Sciences,Zhengzhou450009,China；3Henan AcademyofAgricultural of Scienceand Technology；Xinxiang；Henan453003；China) Abstract：Amolecularmarker basedOilrestrictionand technique enzymesimplesequencerepeat(SSR)， whichshowed usedtofindthedifferencebetweentwovarietiesQtPrHnH$salicina． high polymorphism．Was A from after discrepantfragmentbetween‘xiaohuangli’and‘gaixiandali’wasrecycledpolyacrylamidegel tobe thena was to ofthe electrophoresis designedaccordingsequencefragment．The sequenced．Andprimer ofthe andtheSSR onthetwovarietiesshowedthatthedifferenceWas amplificationprimer primerperformed translatedtoaSSR aSCAR successfully marker(alsomarke0 marker