钩藤总生物碱对高架十字迷宫模型大鼠行为学及脑-第三军医大学学报.DOCVIP

钩藤总碱对焦虑模型行为学和大鼠脑组织单胺类神经递质的影响 李晶晶，汤建林，胡岚岚，周世文，周吉银 （400037 重庆，第三军医大学新桥医院临床药理基地） [摘要] 目的 考察钩藤总生物碱（Rhynchophylla total alkaloidsRTA）抗焦虑。 采用国际通用的高架十字迷宫焦虑动物模型(EPM)观察RTAmg/（kg·d）]、RTAⅡ组[400mg/（kg·d）]、RTAⅢ组[800mg/（kg·d）]三个剂量组对大鼠行为学的影响，用测定EPM大鼠的脑组织内去甲肾上腺素(NE)、多巴胺（DA）5-羟色胺(5-HT)单胺类递质的含量。与对照组比较，RTA组大鼠进入开放臂次数百分比（OE%）和在开放臂滞留时间百分比（OT%）有显著升高（P0.05），脑组织中去甲肾上腺素(NE)、多巴胺（DA）、5-羟色胺(5-HT) 含量（P0.05）。 抗焦虑作用，降低脑组织中去甲肾上腺素(NE)、多巴胺（DA）5-羟色胺(5-HT)含量[关键词] 钩藤总碱高架十字迷宫模型单胺神经递质抗焦虑Effect of the Rhynchophylla total alkaloids on the ethology in the anxiety model and measure monoamine neurotransmitters content in the brain of rats Li Jingjing, Tang Jianlin, Hu Lanlan, Zhou Shiwen, Zhou Jiyin (Base for Clinical Pharmacology,Xinqiao Hospital,Third Military Medical University, Chongqing,400037,China) [Abstract] Objective To investigate the putative anxiolytic-like effects of the Rhynchophylla total alkaloids（RTA). Method Adopt the internationally accepted [通信作者汤建林，:(023E-mail：jltang6317@163.comelevated plus-maze anxiety animal model(EPM) in rats and the anticipatory anxiety experiments in mice to observe the effects of RTAⅠ[200mg/（kg·d）]、RTAⅡ组[400mg/（kg·d）]、RTAⅢ组[800mg/（kg·d）] three dose groups on rats behavior. Then measure Norepinephrine (NE),dopamine (DA) and 5-hydroxytryptamine (5-HT) monoamine neurotransmitters content in rats brain tissues by enzyme-linked immunosorbent assay (ELISA). Results Compared to control group, the percentage of times that rats have entered the open arm (OE%) and percentage of time span that rats have been sticking in the open arms (OT%) were significantly higher (P 0.05) in animals from all three groups applied with different doses of RTA. There was no stress-induced hyperthermia (SIH) in mice of three RTA groups. Norepinephrine (NE),dopamine (DA) and 5-hydroxytryptamine (5-HT) content in rats brain tissues decreased significantly in all three RTA groups(P 0.05). Conclusion: According to rat EMP models and mice anticipatory anxiety experiments, it show