甘蓝型油菜小孢子.pdfVIP

20 10 年 9 月 中国油料作物学报 20 10, 32 ( 3): 349 - 353 Ch inese jou rn al of o il crop sc ien ces DH 1, 2 1 1 1 2 1* 介智靖 , 闫晓红 , 方小平 , 杨 洁, 黄进勇 , 魏文辉 ( 1. , , 430062; 2. , 4 50001) , , , , 2. 5 ~ 3. 5mm , 0. 1m g / L 6- BA , ( doub led haplo id, DH ) 49% , ( 11. 3% ) ( 1. 1% ) ( 27. 3% ) DH ; ; ; ; S565. 403 A 1007 - 9084( 2010) 03- 0349 - 05 Construction of doubled haploid plants and flow detection of their ploidies in B rassica nap us 1, 2 1 1 1 2 1* JIE Zhi- jing , YAN X iao- hong , FANG X iao- p ing , YAN G Jie , HUANG J in - yong , WE IW en - hu i ( 1. D ep artm ent of Genom ics and M olecular B iology, O il Crop s R esearch nstitu te of the Chinese A cademy of A gricultural Sciences/K ey Laboratory of Oil Crop B iology of theM in istry of A g riculture, Wuhan 4 30062, China; 2. D ep artm ent of B ioeng ineering, Zhengzhou University, Zhengzhou 450001, China ) Abstract L arge and sm all seed lines o fB rassica nap us L. w ere u sed fo rm icrospo re culture and haplo id identi fication by flow cytom etry m ethod. The factors effected em bryo produ ction rate w ere invest igated. It w as found that the op tmi al bud m aterial o f 2. 5 to 3. 5mm grew at pr mi ary flow ering period. The liqu id m edium added w ith 0. 1m g /L 6- BA w as able to accelerate the em bryogenesis. A ctivatedcarbon h as no obv ious prom o tion to the oc currence of em bryo. T he flow cytom etry w as found to be qu ick and accurate on plo idy detection. R esu