第一组超分辨成像及应用.精品.ppt

动态成像 · 囊泡运输 · 细胞膜脂筏 · 等等 Mitochondria （线粒体） Figure 1. Simulations. (a) Point spread functions (PSFs) drawn to scale of (i) a confocal microscope (diffraction limited), (ii) a super-resolution (SR) microscope enabling a resolution of 30 nm in the optical plane and conventional resolution along the z-axis, and (iii) a super-resolution microscope enabling isotropic resolution of 30 nm. This resolution was chosen because it can be attained with various instruments and because antibody labeling using a primary and a dye labeled secondary antibody would increase any structure to about this size . (b–d) Simulations on how different labeled mitochondrial structures would appear in the microscopic image. To this end, the simulated data (top: 3D rendering of the data) were convolved with the respective PSFs. Shown are xy and xz sections. The simulated mitochondria have a diameter of 220 nm (b,c; inner membrane) and 280 nm (d; outer membrane). Scale bars: 500 nm. Figure 2. Super-resolution microscopy of protein distributions in mammalian mitochondria. (a) Two-color STORM images showing the interaction between mitochondria (magenta) and microtubules (green) .(b) Two-color STED microscopy of hVDAC3 and hexokinase-I [47]. (c,d) GSDIM and STED imaging of Mic60 (mitofilin) showing the peculiar ordered arrangement of the MICOS clusters . Scale bars: 1000 nm. Jakobs, S., C. A. Wurm: "Super-resolution microscopy in mitochondria" Curr. Opin. Chem. Biol. 20, 9 - 15 Neurobiology （神经生物学） Figure 1. STORM imaging of actin filaments in the axon. (A) Conventional wide-field fluorescence image of actin (green) and the dendritic marker MAP2 (magenta) in a fixed neuron. (B) Three-dimensional STORM image corresponding to the inset outlined by the yellow rectangle in (A), containing axons and devoid of dendrites. The periodic ring-like structure of actin wrapped around the circumference of the axon is clearly apparent. The white boxed insets display the y/z cross sections. Figure 2. Nanocl