[自然科学]Chapter1 Protein sorting.ppt

Advanced Molecular Biology in Cell三部分相对独立又有其关联 Part 1 Protein sorting and vesicle traffic and Techniques in Cell Biology（印莉萍） Chapter1 Protein sorting Chapter2 Membrane vesicle traffic and Mechanisms of Endocytosis Chapter3 Lipid Rafts mediated Exocytosis Chapter4 Molecular Mechanism of Autophagy Chapter5 Techniques in Cellular Biology Test 怎么讲？ 快速浏览“学过”的内容 细致讲解“进展”内容 重点讨论技术方法 考试（需要商量） 考试：三部分各占1/3，总分76分及格 第一部分的考试内容： 听力：5个“上课动画”，放录音，放哪部分，请同学写出那部分的 mean idea。 笔试 1.1 Types and The machinery of protein sorting 1.2 Co-translational Translocation 1.3 Example of my Lab (protein in PM) 1.4 Post-translocation 1.5 Example of my Lab (protein in chloroplast) 1.2 Co-translational Translocation  1.2.1 The role of ER in protein sorting and assembly 1.2.2 Membrane proteins translocation 1.2.3 Lysosome protein sorting 1.2.4 Vacuoles from Biogenesis to Function EXPERIMENTAL： Secretory proteins enter the ER. 利用绿色荧光蛋白GFP示踪分泌蛋白质的分泌途径 Cotranslational Translocation Is Initiatedby Two GTP-Hydrolyzing Proteins CGN ?N-acetylglucosamine a phospho transferase  ?phospodiester glycisidase 1.2.4 Vacuoles from Biogenesis to Function Comparison of different pathways for the delivery of storage proteins to vacuoles in seeds. Plant Vacuoles: from Biogenesis to Function Ds-MxIRT1 lost Fe2+ uptake ability in yeast DsMxIRT1 shows little sensitivity to Cd2+ in yeast DsMxIRT1 showed mis-localization and lost its targeting ability in yeast DsMxIRT1 reduces the iron uptake ability in transgenic Arabidopsis DsMxIRT1 can not target MxIRT1 to the plasma membrane in plant protoplast Mature MxIRT1 seemed to be lack of the N-terminal signal sequence 1.3.1 Transport into mitochondria 1.3.2 Targeting proteins to the plastids 1.3.3 Transport into peroxisomes 1.3.4 Transport in and out of the nucleus The four compartments of a mitochondrion. Import of polypeptides to the chloroplast is acc