罗丹明B分子印迹固相萃取小柱的研制及应用.pdfVIP

24 2 Vol. 24No.2 44 2015 3 CHEMICAL ANALYSIS AND METERAGE Mar. 2015 doi 10.3969j.issn2015.02.013 B * 1 2 2 2 (1. 510260 2. 510006) B B B B B (0.05 mmolL) n 98.24%90.0%95.0%0.9%~1.7% ( =3) B B O652.6A1008 6145(2015)02 0044 04 Preparation and Application of Rhodamine B Molecularly Imprinted Polymer Solid Phase Extraction Column 1 2 2 2 Li Weizhen , Liu Yanling , Wu Qianyu , Zhou Qing (1. Pharmacy Department of No. 1 Hospital for Disabled Soldiers in Guangdong Province, Guangzhou510260, China; 2. Department of Pharmacy, Guangdong Pharmaceutical University, Guangzhou510006, China) AbstractSolid phase extraction (SPE) column of Rhodamine B molecularly imprinted polymer (MIP) was prepared. Rhodamine B MIP was prepared by precipitation polymerization, whose absorption capability was characterized by equilibrium absorption experiment and SPE experiment. The colum was applied to the determination of Rhodamin B in pepper samples. The absorption capability of Rhodamine B MIP was significantly superior to that of NMIP. The extraction rate of MIPSPE to 0.05 mmolL Rhodamin B solution was 98.24% and the recoveries ranged from 90.0% to 95.0%, the relative n deviation of detection results was 0.9% 1.7% ( =3). Rhodamine B MIP presented a good specific recognition to Rhodamine B. SPE column has good selectivity and high extraction rate, which can be applied in food and cosmetics testing fields. K