000001a3栗志文应用药理.docVIP

即溶普洱茶珍不同给予方式对KKAy小鼠降糖效果的影响 栗志文，王根辈，何 莹，曹 晶，张莉华，郭嘉华，马晓慧，朱永宏* （天津天士力集团研究院药理毒理所，天津 300410） [摘要目的比较不同给予方式对于普洱茶珍降糖功效的影响，为适用人群合理饮用普洱茶珍提供理论依据。方法以C57BL/6J小鼠作为对照组，KKAy小鼠随机分为糖尿病模型组、普洱茶珍灌胃组（0.375、0.75、1.5g·kg-1·d-1）、普洱茶珍自由饮用组（0.375、0.75、1.5g·kg-1·d-1）和罗格列酮组8组，连续给药30d，检测小鼠体重、进食量、饮水量、空腹血糖、糖耐量和血清胰岛素等指标。结果灌胃普洱茶珍及自由饮用普洱茶珍均能降低KKAy小鼠进食量，抑制体重增长，减少饮水量。灌胃普洱茶珍1.5g·kg-1·d-1才能产生明显的降低空腹血糖作用，而自由饮用普洱茶珍从0.375g·kg-1·d-1到1.5g·kg-1·d-1剂量范围均能产生明显降低空腹血糖的作用，且饮用后期逐渐呈现明显的量效关系。在糖耐量实验中，相同给药剂量自由饮用普洱茶珍比灌胃普洱茶珍餐后血糖和AUC降低更加明显。两种给药方式在改善胰岛素抵抗方面无明显差异。结论灌胃与自由饮用两种给予方式对于普洱茶珍降糖功效存在一定的影响，自由饮用的给予方式更能发挥普洱茶珍的降糖作用。关键词即溶普洱茶珍、Ⅱ型糖尿病、空腹血糖、糖耐量、胰岛素抵抗、KKAy小鼠Anti-Diabetic Activity of Deepure Bio-Puer Tea In Diabetic KK-Ay Mice with Different Administrations LI ZhiWen, WANG GenBei, HE Ying, CAO Jing, GUO JiaHua, MA XiaoHui, ZHU YongHong* (Pharmacology and Toxicology Department of Tasly RD Institute, Tianjin Tasly Group Co., Ltd., Tianjin 300410, China) [ABSTRACT] OBJECTIVE: To evaluate the anti-diabetic activity of Deepure Bio-Puer Tea (DBPT) by different administration route, and to provide a theoretical basic for application of Deepure Bio-Puer Tea. METHODS: KKAy mice were randomly divided into the model group, DBPT-ig group (0.375、0.75、1.5g·kg-1·d-1) , DBPT-drinking freely group (0.375、0.75、1.5g·kg-1·d-1) and positive control group (Rosiglitazone) and C57BL/6J mice were taken as the normal group. The body weight, food-intake, water-intake, fasting blood glucose, oral glucose tolerance, and the serum levels of insulin were measured. RESULTS: Deepure Bio-Puer Tea with the two kinds of administrations decreased the food-intake and the amount of water drinking, reduced the weight growth of KKAy mice. After DBPT was given to mice by intragastric administration 1.5g·kg-1·d-1 and by drinking with the range of dose from 0.375g·kg-1·d-1 to 1.5g·kg-1·d-1 for 1 week, fasting blood glucose of KK-Ay Mice were decreased significantly. And the obvious dose-effect relation was observed in drinking group from week 2 to 4. Blood glucose con