空心菜浮床对鱼塘水质和微生物多样性的影响.pdf

中国环境科学 2016,36(10) ：3071~3080 China Environmental Science 空心菜浮床对鱼塘水质和微生物多样性的影响 李建柱,侯 杰,张鹏飞,柳尧全,夏闰红,马徐发* (华中农业大学,湖北 武汉 430070) 摘要：为从环境微生物的角度探讨生物浮床的水质调节机理,采用宏基因组学测序技术和生物信息学手段,分析了环境微生物和根系微 生物 群落的多样性 .研究 结果表明 , 鱼菜 共生模式下 优势菌门是变形 菌门 (Proteobacteria) 、蓝细 菌门(Cyanobacteria) 、拟 杆菌门 (Bacteroidetes) 、放线菌门(Actinobacteria) 、绿弯菌门(Chloroflexi)和厚壁菌门(Firmicutes)等,主要菌属是聚球藻属(Synechococcus) 、 hgcI_clade 、CL500 -29_marine_group 、芽孢杆菌属(Bacillus)和分支杆菌属(Mycobacterium)等;空心菜根系微生物群落中最丰富的优势 菌门是变形菌门和蓝细菌门,主要菌属是红细菌属(Rhodobacter) 、噬氢菌属(Hydrogenophaga)和聚球藻属等.研究结果还表明低覆盖率 (3.5%) 的空心菜生物浮床能够增加水体的溶解氧含量, 降低水体中含氮化合物的浓度和改善养殖环境中的微生态平衡,提高有益菌和氮 循环细菌的含量. 关键词：空心菜浮床；环境微生物多样性；水质；16S rDNA 中图分类号：X171.1,S917.1 文献标识码：A 文章编号：1000-6923(2016)10-3071-10 Influence on water quality and microbial diversity in fish pond by Ipomoea aquatica floating-bed. LI Jian-zhu, HOU * Jie, ZHANG Peng-fei, LIU Yao-quan, XIA Run-hong, MA Xu-fa (Huazhong Agricultural University, Wuhan 430070, China). China Environmental Science, 2016,36(10)：3071~3080 Abstract ：In order to investigate the influence on water quality and microbial diversity in intensive fish pond by Ipomoea aquatica floating-bed, and the composition and diversity of microbial communities in the samples were researched based on high throughput sequencing of 16S rRNA gene. The results revealed that the dominant bacteria were phyla Proteobacteria, Cyanobacteria, Bacteroidetes, Actinobacteria, Chloroflexi and Firmicutes, the dominant bacteria were genera Synechococcus, hgcI_clade, CL500 -29_marine_group, Bacillus and Mycobacterium in the Aquaponics System, and the most dominant bacteria of rhizosphere microorganisms were phyla Proteobacteria and Cyanobacteria, the dominant bacteria were genera Rhodobacter, Hydrogenophag