清道夫受体介导脂质蓄积分子机制.pptVIP

(7) The internalization of DiI-AcLDL via SRA mutant: *P0.05,n=3 *P0.05,n=3 * * * * DiI-acLDL DiI-acLDL SR-A expression 10 20 30 40 EGFP SR-A NΔ1-27 NΔ1-27LM 0.12 0.10 0.08 0.06 0.04 0.02 0.00 0 EGFP SR-A NΔ1-27 NΔ1-27LM * * Di-leucine signal motif in the cytoplasmic domain is required for MSR-A-mediated internalization. * Di-leucine signal motif-related endocytosis of Ac-LDL may depend on the clathrin-coat pit. * There might be multiple signal motives in cytoplasmic domain of MSR, which adapts the multiple functions of MSR. Di-Leucine motif may be an distinct motif from the VXFD in SR-A mediated internalization and adhesion. * * Intracellular Accumulation of Lipids via Scavenger Receptors Qi Chen Nanjing Medical University 2005.8 * Formation of foam cell Athersclerosis is a chronic inflammatory reaction caused by macrophages and lymphocytes that uptake the pathogenicity lipids invading the vessel. * SR-A and CD36 are the principal receptors responsible for foam-cell formation Macrophage cell takes up lipid by SR-A * Structure of SR-A Six domains Three types Trimeric molecule * Foam cell formation Mφ adhesion Host defense and innate immunity Adaptive responses Disorders of the nervous system Phagocytosis of apoptotic cells Functions of SR-A * The cytoplasmic domain is the key part of the receptor??? * mediate the internalization of ligands such as ox-LDL participate the regulation of cell adhesion and migration accommodate the expression and transport of receptor link-coupled with downstream of signaling pathway Functions of SR-A cytoplasmic domain: * Cytoplasmic domain： Murine: MTKEMTENQRLCPHEREDADCSSESVKFDARSMTASLP HSTKNGPSVQEKLKSFK (55) Rabbit: MAQWDSFTDQQEDTDSCSESVKFDARSNTALLPPNPK GPPLQEKLKSFK (50) Bovine: MAQWDDFPDQQEDTDSCTESVKFDARSVTALLPPHPKN GPTLQERMKSYK (50) Human: MEQWDHFHNQQEDTDSCSESVKFDARSMTALLPPNPK NSPSL