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Primary structure and transcription of an amplified genetic locus:The CUP1 locus of yeast ABSTRACT Copper resistance in yeast is controlled by the CUP1locus. The level of resistance is proportional to the copy number of this locus, which can be found in up to 15 tandemly iterated copies. To elucidate the molecular mechanisms controlling the amplification and expression of the CUP1locus, we determined its full nucleotide sequence. We have also identified and mapped two transcription units within the basicamplification unit of CUP1 in laboratory yeast strains. One of those transcription units is inducible by copper and encodes a low molecular weight copper binding protein-copper chelatin. The increased production of chelatin, due to both gene amplification and induction of transcription, leads to increased resistance of yeast cells to copper ions. Introduction 1. Location of cup1 locus 2. main character of cup1 locus :copies with its fuction 3. proposal mechanism 4. Ideas of the CUP1 study with the yeast 5.Mainly found in the study: genesequence,structure,and the copper-bind protein,and the comparation with the mamalian MT gene. methods Results 1.Mapping the Transcripts of the CUP1 Locus a Result 1)We have used different portions of the basic repeat unit as hybridization probes to detect transcripts de rived from the CUP1 locus (see Fig.a). 2)After hybridization with probe A, two CUP1-derived RNA species are detected: an abundant transcript of about 500 nucleotides in length and a less abundant transcript of about 1,100 nucleotides in length (Fig. b). Result 3)We conclude that the basic amplified CUP1 unit contains two distinct transcription units. The expression of one unit is regulated by copper. Therefore, it probably codes for the inducible copper binding protein, chelatin whose le
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