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细胞生物化学实验教材Exp 8-cell membrane permeability Furong Gao.pptx
Cell membrane permeability, and micro-scale measurement ;Overview;Contents;To understand the permeability of cell membrane
To know the diffusion rate of different molecules across the cell membrane
;【Principle】;
Diffusion is the spontaneous movement of material from a region of high concentration to a region of low concentration.
The size, polarity, charge, and electrochemical gradient across the membrane determine the rate of diffusion of a molecule across the membrane. ;【Principle】;The red blood cell (RBC) is one of the most studied membrane systems and is therefore used as a model to describe the permeability of membrane to solute.;When hemolysis occurs, the RBC membrane bursts open, releases its hemoglobin, then settles to the bottom of the tube, causing the solution to clear.;Therefore, hemolysis can be used to determine the hypertonic, hypotonic and isotonic concentrations of particular nonpenetrating solutes and the hemolysis time can be used as an indicator of the diffusion rate for particular penetrating solutes. ;【Procedure】;【Procedure】;Note: label all tubes or beakers you used in this experiment.;Micro-scale measurement;Micro-scale measurement;Ocular micrometer is a glass disk with a scale engraved on the surface. It is placed into one eyepiece.
A stage micrometer is a microscope slide with a scale etched on its surface. A typical micrometer scale is 1 mm long and divided into 100 divisions. Each division is 0.01mm (10μm).;ocular micrometer;Calibration of ocular micrometer;【Procedure】;Make mouth mucous membrane epithelial cells specimen;Human mouth mucous membrane epithelial cells;Solution;Number of tube;Name of figure;4. Calculate the length of each division of ocular micrometer at low power (10 X) and high power (40 X);5. Calculate the size of epithelial cells ;What’s the physiologic characteristic of red cells?
What’s the characteristic of cell membrane?
If red blood cells are placed in a hypertonic solution of penetrating molecules, what will occur
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