论文审查--杨振昌老师.docVIP

論文審查--化學性危害預防與評估(含工業毒物) 第一篇 姓名 张华 Email ghd1998@126.com 公司名稱 職稱 投稿類型 論文標題 大剂量N-乙酰半胱氨酸对矽肺动物模型作用的研究 口頭報告作者 中文摘要 大剂量N-乙酰半胱氨酸对矽肺动物模型作用的研究 张华1，尹刚2，姜昊3，张春玲4 1、山东省青岛市中心医院，职业病科，2、山东省青岛市中心医院心内科，3、山东省千佛山医院神经内科，4、山东省青岛市中心医院呼吸内科 摘要：目的 研究大剂量N-乙酰半胱氨酸在减轻矽肺纤维化中的作用 方法 我们在建立矽肺动物模型前后分别给予乙酰半胱氨酸（NAC）（500 mg·kg-1）灌胃干预治疗。肺组织纤维化评分和羟脯氨酸(HYP)、丙二醛(MDA)的含量分别被测定。TNF-(免疫组化半定量评估。用ELISA方法测定支气管肺泡灌洗 (BALF)和血清中的肿瘤坏死因子-((TNF-(), 白细胞介素-8 (IL-8)和超敏C-反应蛋白(hsCRP)的含量。结果 大剂量NAC治疗组的肺纤维化评分、肺匀浆HYP和MDA的含量明显低于模型组(P0.01)。NAC能减轻二氧化硅所致BALF和血清中的炎症因子（TNF-α，IL-8，hsCRP）的水平（P0.05)。结论 矽肺纤维化的发生和发展和氧化、炎症反应密切相关。在早期服用大剂量NAC科减轻氧自由基的激活和炎症相关因子的活性，从而减轻肺纤维化，此可作为矽肺纤维化潜在的治疗手段。 关键词：二氧化硅，大剂量N-乙酰半胱氨酸，纤维化，细胞因子 英文摘要 High-dose N-acetylcysteine decreases silica-induced lung fibrosis in rat Zhang Hua1，Yin Gang2，Jiang Hao3，Zhang Chunling4 1Department of Occupational Disease, Qingdao Central Hospital, Shandong, China；2Department of Cardiology, Qingdao Central Hospital, Shandong, China；3 Department of Neurology, Qianfoshan Hospital, Shandong, China；4Department of Respiratory Medicine, Qingdao Central Hospital, Shandong, China Abstract: Purpose: We studied the potential of high-dose N-acetylcysteine (NAC) in attenuating silica-induced pulmonary fibrosis. Methods: We used a silica-exposed rat model of rats treated orally with 500 mg·kg-1 NAC before and after silica administration. The fibrotic score and content of hydroxyproline (HYP) and malondialdehyde (MDA) were assessed in lung tissue. Bronchoalveolar fluid (BALF) or serum levels of tumor necrosis factor ( (TNF-(), interleukin 8 (IL-8) and high-sensitivity C-reactive protein (hsCRP) were assessed by ELISA and TNF-( localization was determined by immunohistochemistry. Results: The silica-induced rat model showed inflammation and fibrosis. In silica-treated tissue, the fibrosis score was lower with high-dose NAC than in controls (P0.01). The content of HYP and MDA was lower with NAC treatment than controls at all times (all P0.01). NAC decreased the silica-increased levels