金属硫蛋白对急性化学性肝损伤保护作用研究.docVIP

金属硫蛋白对急性化学性肝损伤保护作用研究 　　（1.温州医学院附属第一医院，浙江 温州 325027；?2.吉林大学第一医院，吉林 长春 130021；3.哈尔滨春源生物科技开发有限公司， 黑龙江 哈尔滨 150036）?? 　　 　　摘要： ［目的］ 研究含金属硫蛋白（metallothionein，MT）蛋奶粉对CCl4致大鼠急性肝损伤的保护作用。 ［方法］ 70只Wistar大鼠随机分为正常对照组10只、模型组20只、低和高剂量MT蛋奶粉治疗组各20只。低和高剂量MT蛋奶粉治疗组大鼠以含MT蛋奶粉灌胃1周，然后对除正常对照组以外的大鼠复制CCl4肝损伤模型，于造模当日，正常对照组和模型组大鼠用蒸馏水，治疗组用含MT蛋奶粉连续灌胃2周后，断头处死大鼠，观察各组大鼠血清酶类含量的变化和肝脏过氧化物质和抗氧化酶类（SOD、CAT）和GSH含量的变化。 ［结果］ 与正常对照组相比，模型组大鼠血清中ALT、AST、GGT和ALP含量显著升高（P4 induced liver damage in rats. 　　 ［Methods］ Seventy Wistar rats were divided into four groups. They were normal control (n=10), liver damage model group (n=20), low dose MT egg milk group (n=20) and high dose MT egg milk group (n=20). The rats excluding the normal control and model group were given with MT egg milk by intragastric administration for one week, and the normal and model group rats were given normal saline by intragastric administration at the same time. All the rats except those in the normal control group were injected with CCl4 to induce hepatic injury. On this day, the rats in the normal and model group were given normal saline by lavage, and the rats in the MT egg-milk group were given different doses of egg-milk by lavage for 2 weeks. Their serum and livers were then collected for study as follows.Liver marker enzymes (AST, ALT, ALP and GGT), lipid peroxidation markers in liver and anti-oxidative enzymes (SOD and CAT), and non-enzymatic antioxidant (GSH) in liver were measured. 　　 ［Results］ As compared with those in the normal control group, AST, ALT, ALP and GGT levels in the model groups increased significantly (P 4 along with MT egg-milk decreased the MDA content significantly and increased the SOD activities and GSH content of liver towards normal level (P 4 induced oxidative damage in rats. ? 　　Key words: Metallothionein; Egg-milk; Acute liver damage; Antioxidative 　　??? 　　金属硫蛋白（metallothionein, MT）发现于1957年，是广泛存在于生物界的一类低分子量、高金属含量、无芳香氨基酸、无组氨酸、富含半胱氨酸的金属结合蛋白，是目前唯一被确定在细胞内重金属代谢中具有明确作用的蛋白质。其功能独特，具有广泛的生物学效应，如抗